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pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

Plasmid

#62978

Purpose

Expresses myc-tagged mouse βTrCP residues 292-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Tags

myc

Expression

Mammalian

Mutation

codes for residues 292-569

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-RFP-UCR1C

Plasmid

#67582

Purpose

Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Tags

FLAG and TagRFP

Expression

Mammalian

Mutation

Amino acid residues 80-136 of PDE4D3

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
Mc4r->M71-IRES-tauGFP ACNF TV

Plasmid

#105072

Purpose

Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-333 of Mc4r and an IRES-tauGFP followed by ACNF cassette

Depositor

Peter Mombaerts

Article

Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.

Insert

Mc4r->M71-IRES-tauGFP-ACNF (Mc4r Mouse)

Use

Mouse Targeting

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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DGKG gRNA (BRDN0001149479)

Plasmid

#77454

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKG

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKG (DGKG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKG gRNA (BRDN0001145017)

Plasmid

#77455

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKG

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKG (DGKG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKG gRNA (BRDN0001147060)

Plasmid

#77456

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKG

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKG (DGKG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACA gRNA (BRDN0001144986)

Plasmid

#75515

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACA gRNA (BRDN0001145395)

Plasmid

#75516

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACA gRNA (BRDN0001145877)

Plasmid

#75517

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACA gRNA (BRDN0001148333)

Plasmid

#75518

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgPTPN11-1

Plasmid

#253090

Purpose

CRISPRi single-guide against PTPN11

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

PTPN11 (PTPN11 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-AtRBX1-T7-HsSKP1-T7-AtCUL1-S

Plasmid

#228944

Purpose

Expression AtRBX1-T7, HsSKP1-T7 and AtCUL1-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtRBX1 (RBX1 Mustard Weed)
HsSKP1 (SKP1 Human)
AtCUL1 (CUL1 Mustard Weed)

Tags

S-Tag and T7

Expression

Bacterial

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA WT

Plasmid

#249271

Purpose

Expression of PKA (PRKACA) wild type

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA R135W

Plasmid

#249274

Purpose

Expression of PKA R135W mutant (PRKACAR135W)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

R135W

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA E171K

Plasmid

#249275

Purpose

Expression of PKA E171K mutant (PRKACAE171K)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

E171K

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA T49I

Plasmid

#249272

Purpose

Expression of PKA T49I mutant (PRKACAT49I)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

T49I

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA E128K

Plasmid

#249273

Purpose

Expression of PKA T128K mutant (PRKACAE128K)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

E128K

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pET-28a(+) PCMT1 H58 A214

Plasmid

#246496

Purpose

Recombinant protein expression of PCMT1 H58 A214

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 (PCMT1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Threonine 58 mutated to Histidine and Valine 214 …

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A

Plasmid

#244492

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 SHP2 E252A

Plasmid

#244491

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Glutamate 252 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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