We narrowed to 14,787 results for: Lif

1. pDRFLIP38

   Plasmid

   #65516

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-mRuby3

   Plasmid

   #179730

   Purpose

   Mammalian expression of mRuby3 from the EF1 promoter

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+Paxillin-mKate

   Plasmid

   #105974

   Purpose

   in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

   Depositor

   Caren Norden

   Article

   Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

   Insert

   Paxillin
   

   Use

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nsp2Cas9_AAV

   Plasmid

   #192143

   Purpose

   Expresses Nsp2Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nsp2Cas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSIIbsr-GCaMP6s

   Plasmid

   #184712

   Purpose

   Calcium biosensor

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET303-colE1TR

   Plasmid

   #155181

   Purpose

   Expresses colicin E1 residues 1-364

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   colicin E1 residues 1-364
   

   Use

   Tags

   C-terminal 6x Histidine tag

   Expression

   Bacterial

   Mutation

   truncation of colicin E1 including 1-364

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDK521

   Plasmid

   #134204

   Purpose

   Baculovirus expresion of Astrin, LC8, and SKAP

   Depositor

   Iain Cheeseman

   Article

   Kern et al Elife. 2017 Aug 25;6. pii: 26866. doi: 1

   Insert

   Astrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. BFP-Rab5CA (Q79L)

   Plasmid

   #205705

   Purpose

   Mammalian expression of BFP-tagged mutant Rab5CA

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   RAB5A (RAB5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q79L

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. DmTmem63

   Plasmid

   #136598

   Purpose

   Expresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cells

   Depositor

   Ardem Patapoutian

   Article

   Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

   Insert

   DmTMEM63
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFUChW nontargeting shRNA

    Plasmid

    #78522

    Purpose

    Lentiviral vector encoding shRNA and mCherry reporter

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    mCherry
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-jAspSnFR3-mRuby3-NLS

    Plasmid

    #203494

    Purpose

    Nuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    jAspSnFR3-mRuby3-NLS
    

    Use

    Lentiviral

    Tags

    Nuclear localization signal and mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 53BP1 N-terminal sgRNA

    Plasmid

    #207091

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGGGAGCAGATGGACCCTAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR Clover-VISH2

    Plasmid

    #188629

    Purpose

    Fluorescent reporter for SLP76/VISH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    VISH2
    

    Use

    Lentiviral

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPBbsr2-cPLA2-mEGFP

    Plasmid

    #184718

    Purpose

    Exogenous cPLA2WT

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    cPLA2-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-Paxillin DN

    Plasmid

    #186153

    Purpose

    For expressing paxillin variants in mammalian cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PXN (PXN Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Paxilln residues 1-321 added to N-terminus of WT …

    Promoter

    cmv

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCZGY3163

    Plasmid

    #101248

    Purpose

    GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    gfp11::rps-18 (rps-18 Synthetic, Nematode)
    

    Use

    Gateway

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-mScarlet-TEV-Strep-His

    Plasmid

    #210823

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mScarlet
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. NES-iRFP-P4Mx1

    Plasmid

    #108144

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):iRFP:L. penomophila SidM(546-647)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2Aneo-tdTomato-CAAX

    Plasmid

    #170284

    Purpose

    Encoding membrane-anchored tdTomato.

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    tdTomato-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM1-PCYT2

    Plasmid

    #81074

    Purpose

    Expresses PCYT2 in mammalian cells

    Depositor

    Erin O'Shea

    Article

    Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

    Insert

    phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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