We narrowed to 5,570 results for: donor

1. pSLS.492

   Plasmid

   #184957

   Purpose

   Test effect of a1/a2 length on editing rpoB using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
   

   Expression

   Bacterial

   Mutation

   rpoB donor T1534C, a1/a2 length extended to 22 bp

   Promoter

   T7/lac

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem

   Plasmid

   #229794

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMC_mNG2(11)_BSDminus_F2

   Plasmid

   #184164

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-Puro cTnT FUCCI

   Plasmid

   #136935

   Purpose

   donor plasmid for cardiac-specific FUCCI expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   Troponin T promoter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   cTnT

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPP3373

   Plasmid

   #128718

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopQ1-1
   

   Expression

   Bacterial

   Mutation

   Synonymous point mutation in the wobble position…

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PDX1-2A-eGFP

   Plasmid

   #66964

   Purpose

   PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   pancreatic and duodenal homeobox 1 (PDX1 Human)
   

   Use

   Unspecified

   Tags

   2A-eGFP

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. ppk301-T2A-QF2 HDR plasmid

   Plasmid

   #130667

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
   

   Use

   Donor template

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem

   Plasmid

   #229792

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

   Plasmid

   #139484

   Purpose

   PCR template for mEGFP gene knock-in donor vector

   Depositor

   Kazuhiro Aoki

   Article

   Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

   Insert

   mEGFP-loxP-P2A-dTKneo-pA-loxP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid

    #227245

    Purpose

    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

    Depositor

    Jae Seong Lee

    Article

    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

    Inserts

    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1α and pTRE3G

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBbsr2-Lifeact-miRFP703

    Plasmid

    #178530

    Purpose

    Expresses Lifeact-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Lifeact-miRFP703
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Halo-TCAB1 HRD

    Plasmid

    #207535

    Purpose

    Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-PKCb1-mEGFP-N1

    Plasmid

    #112265

    Purpose

    ITRACKbeta/IDOCKSbeta Donor construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c beta (Prkcb Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCPP3416

    Plasmid

    #128720

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopT1-1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPP5060

    Plasmid

    #128716

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

    Insert

    shcN-hopN1
    

    Expression

    Bacterial

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCPP6450

    Plasmid

    #128731

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

    Insert

    HopAD1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2-CAG-fGFP

    Plasmid

    #108714

    Purpose

    Transposon donor plasmid for long-term expression of membrane targetted GFP

    Depositor

    Madeline Lancaster

    Article

    Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

    Insert

    EGFP
    

    Tags

    6xHis and farnesylation signal

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG_mIGF1R_EGFP

    Plasmid

    #199629

    Purpose

    mutant FRET donor for IGF1R sensor

    Depositor

    Ryohei Yasuda

    Article

    Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

    Insert

    mIGF1R (IGF1R Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y1316F

    Promoter

    CAG

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLHDR02_EGFP_tCD8

    Plasmid

    #124061

    Purpose

    Construction of 1st donor for scarless genome editing

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Inserts

    EGFP
    tCD8
    

    Use

    Mouse Targeting; Human targeting

    Promoter

    Human UbC promoter modified

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRepair-MPP8_mAID-P2A-BFP

    Plasmid

    #175036

    Purpose

    Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    mAID-P2A-BFP
    

    Use

    Hdr donor template

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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