We narrowed to 7,632 results for: ef1a

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AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

Plasmid

#228364

Purpose

mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Enascas12a nuclease and en…

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCEFLmGFP-Gli2

Plasmid

#37672

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Tags

mGFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pJL96

Plasmid

#243684

Purpose

Stably expresses cytosolic LOV-BirA

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

LOV-BirA-GFP

Use

Lentiviral

Tags

3xFLAG

Promoter

EF1a

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-PEST

Plasmid

#204766

Purpose

GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

EGFP-PEST-m6Alinker-DHFR

Mutation

mutated all RACs in EGFP to RGC/RCC

Promoter

CMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV-H2B-eGFP

Plasmid

#91776

Purpose

Expresses H2B protein tagged with eGFP

Depositor

Maria Pia Cosma

Article

Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

Insert

Human Histone H2B / eGFP (H2BC11 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCF221

Plasmid

#121669

Purpose

U6-sgRNA EF1a-mCherry

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

mCherry

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-dAPEX2

Plasmid

#117174

Purpose

Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

dAPEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX_cpCys203

Plasmid

#247756

Purpose

Lentiviral expression of the cpCys203 FRET-based cysteine sensor in mammalian cells

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

cpCys203 Cysteine Sensor

Use

Lentiviral

Tags

ECFP and Venus

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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ptwB

Plasmid

#48735

Purpose

Tandem dual tomato plasmid

Depositor

John Schiller, Roger Tsien

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

Tandem dual tomato

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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p23-DDX21 WT

Plasmid

#128803

Purpose

overexpression of DDX21 in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 WT

Tags

flag

Expression

Mammalian

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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RAB14 WT

Plasmid

#61493

Purpose

to overexpress wild type RAB14. Vector includes GFP driven by hUbC promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
IDP456

Plasmid

#87002

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

barcode landing site-mCherry-4xboxB

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCEFL3xHAmGli2

Plasmid

#37671

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pEF-APEX2-NLS

Plasmid

#140281

Purpose

Expression of APEX2-NLS

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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IDP432

Plasmid

#87000

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

SNAP-Nlg1AB-1xλN(i)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMB1: All in one SpCas9 expression and sgRNA cloning backbone

Plasmid

#228363

Purpose

mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-dfROX

Plasmid

#137170

Purpose

Lentiviral transfer vector for expression of roGFP2 and HyPer Red

Depositor

Shaun McCullough

Article

Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

Insert

roGFP2-2A-HyPer Red

Use

Lentiviral

Promoter

EF1a

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD

Plasmid

#170124

Purpose

Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Sico-Red

Plasmid

#84882

Purpose

Cre-inducible shRNA

Depositor

Eun Mi Hwang

Article

Jung et al Exp Neurobiol. 2016 Jun;25(3):120-9. doi

Insert

Sico-Red

Use

AAV

Tags

mCherry

Promoter

EF1a and CMV

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-ReaChR-P2A-dTomato

Plasmid

#114368

Purpose

expresses a Cre-dependent red light-shifted channelrhodopsin variant

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

ReaChR-P2A-dTomato

Use

AAV

Tags

dTomato

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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