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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2501 - 2520 of 5523 results

  1. pAL005

    Plasmid
    #233865
    Purpose
    MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Depositor
    Jeff Hasty
    Publication
    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102
    Insert
    pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Expression
    Yeast
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    233865-plasmid-map-sequence-id-468718

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  6. pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227923
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RPL36 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227923-plasmid-map-sequence-id-456037

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  7. pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227907
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227907-plasmid-map-sequence-id-452503

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  8. pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227910
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227910-plasmid-map-sequence-id-456010

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  9. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227904
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227904-plasmid-map-sequence-id-452481

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  10. pCHW90004

    Plasmid
    #222436
    Purpose
    Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.
    Depositor
    Chih-Hang Wu
    Publication
    Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi
    Insert
    CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Available since
    Nov. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222436-plasmid-map-sequence-id-452566

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  13. pMSCV-BLU-VIPR

    Plasmid
    #220497
    Purpose
    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Publication
    Pulgarin et al Nucleic Acids Res. 2025 Mar 20;53(6):gka
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    220497-plasmid-map-sequence-id-442817

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  14. DVA_p15A_AF

    Plasmid
    #217604
    Purpose
    Destination vector with ampicillin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.
    Depositor
    Wei Huang
    Publication
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217604-plasmid-map-sequence-id-442590

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  15. DVC_p15A_AF

    Plasmid
    #217588
    Purpose
    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Publication
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217588-plasmid-map-sequence-id-442568

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  16. MoBiFC

    Plasmid kit
    #1000000222
    Purpose
    Modular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein interactions in plants.
    Depositor
    Benjamin Field
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Plant Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  20. pNK2657

    Plasmid
    #219741
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Publication
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    hispidin- synthase from Mycena citricolor
    Use
    Synthetic Biology
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219741-plasmid-map-sequence-id-437095

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Showing: 2501 - 2520 of 5523 results