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Showing: 2521 - 2540 of 18816 results

  1. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Use
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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  4. pBIG2abc_hsHAUScomplex_FL

    Plasmid
    #202203
    Purpose
    Baculoviral transfer vector to co-express 8 subunits of human augmin complex
    Depositor
    Thomas Surrey
    Article
    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109
    Inserts
    HAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)
    Use
    Tags
    -mGFP-TEV-TwinStrep and His-tag (6x)
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    202203-plasmid-map-sequence-id-407808

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  5. pGLU_108

    Plasmid
    #225789
    Purpose
    RS421 library variant 13166
    Depositor
    Nili Ostrov
    Insert
    13166_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225789-plasmid-map-sequence-id-447022

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  6. pGLU_90

    Plasmid
    #225804
    Purpose
    RS421 library variant 13166
    Depositor
    Nili Ostrov
    Insert
    13166_[Himar]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225804-plasmid-map-sequence-id-446933

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  7. pGLU_72

    Plasmid
    #225774
    Purpose
    RS421 library variant 13166
    Depositor
    Nili Ostrov
    Insert
    13166_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225774-plasmid-map-sequence-id-446930

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  8. pORFE0010

    Plasmid
    #112074
    Purpose
    3xmVenus module CT
    Depositor
    Christian Chevalier
    Insert
    3xmVenus
    Use
    Tags
    Expression
    Plant
    Mutation
    Ser to Thr at amino acid 63 and Phe to Leu at pos…
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112074-plasmid-map-sequence-id-217639

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  9. pGLU_65

    Plasmid
    #225768
    Purpose
    This is the native promoter from the aac(3)-Ia gentamicin resistance cassette in IDT backbone
    Depositor
    Nili Ostrov
    Insert
    Paac(3)-Ia_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225768-plasmid-map-sequence-id-447050

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  10. pSB3K3-101-phzAG

    Plasmid
    #165617
    Purpose
    Constitutive expression of phzAG operon for phenazine-1-carboxylic acid production
    Depositor
    Martin Buck
    Insert
    phzABCDEFG
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    165617-plasmid-map-sequence-id-331403

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  11. pET41-FeoB

    Plasmid
    #216753
    Purpose
    Overexpression of FeoB for biological characterisation
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Wild type
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    216753-plasmid-map-sequence-id-447881

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  12. pGlu57

    Plasmid
    #211560
    Purpose
    Amber Suppressor tRNA for Glutamate
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNAGlu
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211560-plasmid-map-sequence-id-438928

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  13. pGly57

    Plasmid
    #211561
    Purpose
    Amber Suppressor tRNA for Glycine
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNAGly
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211561-plasmid-map-sequence-id-438930

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  14. pHis57

    Plasmid
    #211562
    Purpose
    Amber Suppressor tRNA for Histidine
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNAHis
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211562-plasmid-map-sequence-id-438922

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  15. pLeu57

    Plasmid
    #211563
    Purpose
    Amber Suppressor tRNA for Leucine
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNALeu
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211563-plasmid-map-sequence-id-417239

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  16. pLys57-1

    Plasmid
    #211564
    Purpose
    Amber Suppressor tRNA for Lysine
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211564-plasmid-map-sequence-id-438925

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  17. pPhe57

    Plasmid
    #211568
    Purpose
    Amber Suppressor tRNA for Phenylalanine
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNAPhe
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211568-plasmid-map-sequence-id-438920

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  18. pPro57

    Plasmid
    #211569
    Purpose
    Amber Suppressor tRNA for Proline
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNAPro
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211569-plasmid-map-sequence-id-438933

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  19. pSer57

    Plasmid
    #211570
    Purpose
    Amber Suppressor tRNA for Serine
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNASer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211570-plasmid-map-sequence-id-421061

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  20. pAla57

    Plasmid
    #211556
    Purpose
    Amber Suppressor tRNA for Alanine
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNAAla
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211556-plasmid-map-sequence-id-417300

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Showing: 2521 - 2540 of 18816 results