We narrowed to 4,028 results for: LYC;

1. pN7HELIX

   Plasmid

   #190662

   Purpose

   Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   CMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. R57C10-Flp2

   Plasmid

   #63172

   Purpose

   R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   yeast wild type containing a Glycine at amino acid 5 of the protein sequence
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET21a-GlyRS-His

   Plasmid

   #124110

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pN7CAST

   Plasmid

   #190661

   Purpose

   Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHYX137

   Plasmid

   #202814

   Purpose

   Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

   Depositor

   Jean-Félix Dallery

   Article

   Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScPCK1

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHYX138

   Plasmid

   #202815

   Purpose

   Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

   Depositor

   Jean-Félix Dallery

   Article

   Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScADH2

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV/myc/Nuc-hOGG1

   Plasmid

   #18709

   Depositor

   David Sidransky

   Article

   Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

   Insert

   8-oxoguanine DNA glycosylase (OGG1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pPGKpuro

   Plasmid

   #11349

   Depositor

   Rudolf Jaenisch

   Article

   Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

   Insert

   pgk-1 5' and 3' regions (Pgk1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-CEPT1

   Plasmid

   #119079

   Purpose

   Visualize localization of ER enzyme CEPT1, which converts diacylglycerol precursors to phosphatidylethanolamine or phosphatidylcholine

   Depositor

   Gia Voeltz

   Article

   English et al Nat Cell Biol. 2013 Feb;15(2):169-78. do

   Insert

   CEPT1 (CEPT1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCMV_SARS-CoV-2_Sdel18

    Plasmid

    #207307

    Purpose

    Mammalian expression of SARS2 coronavirus S protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    SARS-CoV2_S (S SARS2 coronavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV/myc/mito-hOGG1

    Plasmid

    #18706

    Depositor

    David Sidransky

    Article

    Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

    Insert

    8-oxoguanine DNA glycosylase (OGG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pGolden-Neo

    Plasmid

    #51422

    Purpose

    Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPLP2S-mCf

    Plasmid

    #229986

    Purpose

    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNAS1B

    Plasmid

    #61968

    Purpose

    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Serine 65 to Glycine (GGT), Serine 72 to Alanine …

    Promoter

    PBAD and PLtetO

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-ConVERGD-N2cG-WPRE

    Plasmid

    #218757

    Purpose

    Provides AND intersectional (Cre+Flp) expression of N2C glycoprotein

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    N2cG
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Depositor Comments

    Promoter

    hSyn

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE655

    Plasmid

    #153253

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. LT2_12_GlyRNA

    Plasmid

    #177710

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. enz.083-NMT1

    Plasmid

    #154047

    Purpose

    Expresses NMT1 in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    NMT1 (N-myristoyltransferase 1) (NMT1 Human)
    

    Use

    Tags

    His, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRT802

    Plasmid

    #68113

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    aph11
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. mOtop1_pcDNA3

    Plasmid

    #114677

    Purpose

    This construct was used to express mouse Otop1 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 1 (Otop1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SNP (rs49325632): Glycine 396 to Alanine

    Promoter

    CMV

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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