We narrowed to 6,235 results for: nls

1. pLVX-Puro-mRuby-WDR34

   Plasmid

   #66599

   Purpose

   Expresses WDR34 as a fusion with monomeric mRuby

   Depositor

   David Stephens

   Article

   Asante et al J Cell Sci. 2014 Nov 1;127(21):4774-87.

   Insert

   WDR34 (WDR34 Human)
   

   Use

   Lentiviral

   Tags

   mRuby

   Expression

   Mammalian

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Sus1/PET32a

   Plasmid

   #26963

   Depositor

   Cynthia Wolberger

   Article

   Samara et al Science. 2010 May 21. 328(5981):1025-9.

   Insert

   Sus1 (SUS1 Budding Yeast)
   

   Tags

   His6 and Trx

   Expression

   Bacterial

   Mutation

   with TEV site to cleave the N-terminal his+Trx tag

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCHW00015

   Plasmid

   #222446

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   VP64-NLS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCHW00014

   Plasmid

   #222445

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   VP16-NLS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Efg1-ΔDBD-Q443P

   Plasmid

   #216401

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Q443P
   

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Glutamine 443 to Proline, and deleted ami…

   Available Since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV/CMV-SCLM

   Plasmid

   #216758

   Purpose

   AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon

   Depositor

   George Augustine

   Insert

   SuperClomeleon followed by WPRE and human growth hormone polyA terminator
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   CMV (cytomegalovirus)

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ300

   Plasmid

   #205414

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter

   Depositor

   Yiping Qi

   Article

   Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

   Insert

   Ev1Cas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS (N terminal on insert) NLS (C terminal on ins…

   Expression

   Plant

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. JAB3477

   Plasmid

   #190416

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XPhlFO-3XAmtRO::TetR-NLS-19St)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   6XPhlFO-3XAmtRO::TetR-NLS-19St
   

   Expression

   Plant

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. JAB3617

   Plasmid

   #190427

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (pro35S::TetR-NLS-19St)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   pro35S::TetR-NLS-19St
   

   Expression

   Plant

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. JAB3017

    Plasmid

    #190399

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::McbR-NLS-19St)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proG1090::McbR-NLS-19St
    

    Expression

    Plant

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. JAB2964

    Plasmid

    #190398

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XAmtRO-2XPhlFO::TetR-NLS-19St)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    6XAmtRO-2XPhlFO::TetR-NLS-19St
    

    Expression

    Plant

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC0_3_Mxi1

    Plasmid

    #163715

    Purpose

    Plasmid encoding Mxi1 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    Mxi1+SV40 NLS
    

    Use

    Synthetic Biology; Moclo vector level 0 (type 4a)

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Himar-dCas9-mammalian

    Plasmid

    #138414

    Purpose

    Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Tags

    3xFLAG, 6xHis, and SV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMM767

    Plasmid

    #133604

    Purpose

    Part Plasmid for dCas9 NLS, Part 3(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    dCas9 NLS
    

    Use

    Synthetic Biology

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM768

    Plasmid

    #133605

    Purpose

    Part Plasmid for dCas9 NLS, Part 3b(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    dCas9 NLS
    

    Use

    Synthetic Biology

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMM769

    Plasmid

    #133606

    Purpose

    Part Plasmid for dCas9 NLS, Part 3a(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    dCas9 NLS
    

    Use

    Synthetic Biology

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Intersectin SH3 domain A

    Plasmid

    #47396

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain A
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    SH3 domain A

    Promoter

    tac

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pErCas12a EZ Clone

    Plasmid

    #132641

    Purpose

    Expresses ErCas12a and an sgRNA to target a region of interest

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    AAV and CRISPR

    Tags

    3x HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q926R (Please see depositor comments) and BsaI si…

    Promoter

    CMV Promoter

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH127

    Plasmid

    #162483

    Purpose

    SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

    Depositor

    Bob Goldstein

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    

    Use

    CRISPR

    Tags

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

    Expression

    Worm

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJH130

    Plasmid

    #162484

    Purpose

    SEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

    Depositor

    Bob Goldstein

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    

    Use

    CRISPR

    Tags

    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

    Expression

    Worm

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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