We narrowed to 10,582 results for: plasmids 101

1. pSN33

   Plasmid

   #100118

   Purpose

   expresses a chimeric KLF4-repressor fusion

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCherry-P2A-KLF4 fused to full length HP1 alpha (KLF4, CBX5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSN29

   Plasmid

   #100114

   Purpose

   expresses a chimeric cMYC-repressor fusion

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCerulean-P2A-cMYC fused to full length HP1 alpha (MYC, CBX5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSFI-myr-akt1

   Plasmid

   #49186

   Purpose

   cDNA of Akt1 with myristyolation signal in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a-tagBFP-bGH

   Plasmid

   #235257

   Purpose

   tagBFP expression to serve as a "filler" plasmid for transfections

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   tagBFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations to remove BsaI sites in tagBFP

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hy_pMT dati MCS Exon

   Plasmid

   #110119

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   dati (dati Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hy_pMT dati Exons 1-3

   Plasmid

   #110113

   Purpose

   Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   dati (dati Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSynapsin.TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

   Plasmid

   #187101

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-SF-iGluSnFR
   

   Use

   AAV

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pc3XB-ZF2

   Plasmid

   #13101

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   ZF2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. JD108

   Plasmid

   #229494

   Purpose

   Adenoviral DeltaF6 plasmid variant devoid of fiber, U exon and pVIII sequences

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   multiple genes
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7-VEE-OKS-iG

    Plasmid

    #58974

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-HIF1 alpha

    Plasmid

    #21101

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCm C-term MBP NikJC199U

    Plasmid

    #174365

    Purpose

    Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, MBP, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-fugu SLC2A4 promoter

    Plasmid

    #65101

    Purpose

    Luciferase reporter construct under the fugu (Takifugu rubripes) SLC2A4 promoter.

    Depositor

    Josep Planas

    Article

    Marin-Juez et al PLoS One. 2013 Nov 18;8(11):e80628. doi:

    Insert

    SLC2A4 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

14. G1088E_pLenti-CMV-mNeonGreen-2A-HygroR

    Plasmid

    #216279

    Purpose

    Green and hygromycin resistant lentiviral vector plasmid for multiplexed receptor pseudovirus experiments

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2024 May 20;20(5):e1012044.

    Insert

    mNeonGreen-2A-Hpt
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217478

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1s
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217479

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1b
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCm N-term SMT3 NikJC199U

    Plasmid

    #174210

    Purpose

    Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, SMT3, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCm C-term SMT3 NikJC199U

    Plasmid

    #174362

    Purpose

    Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, SMT3, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCm N-term MBP NikJC199U

    Plasmid

    #174364

    Purpose

    Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-human OCT4 PE-SV40-Luc

    Plasmid

    #52415

    Purpose

    Luciferase reporter plasmid for PE OCT4 enhancer activity

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    Proximal element of hOCT4 promoter (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge