We narrowed to 87,726 results for: lias

1. pET28a(+)-SUMO-A'-l4-G'

   Plasmid

   #209124

   Purpose

   Bacterial expression of CC-GEMS ligand SUMO-A'-l4-G'

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-TO-HA-SH3BP1 (RNAi-res)

   Plasmid

   #136645

   Purpose

   Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

   Depositor

   Karl Matter, Maria Balda

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1 (RNAi resistant) (SH3BP1 Human)
   

   Use

   Tags

   HA (MQDLPGNDNSTAG)

   Expression

   Mammalian

   Mutation

   GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-HA-hTRAF3 1-448

   Plasmid

   #44033

   Depositor

   Shao-Cong Sun

   Article

   Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

   Insert

   TNF receptor-associated factor 3 (TRAF3 Human)
   

   Use

   Tags

   1xHA

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-448; V274D and S393G

   Promoter

   Available since

   April 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKMD106h - intein-Cas9(C574)

   Plasmid

   #64191

   Purpose

   Expresses intein-Cas9(C574) in mammalian cells

   Depositor

   David Liu

   Article

   Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

   Insert

   mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
   

   Use

   CRISPR

   Tags

   NLS - 3x FLAG (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-NLS-gs10-cGR2176

   Plasmid

   #188258

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cGR2176
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pegRNA_HEK4_20-7

   Plasmid

   #201974

   Purpose

   Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   pegRNA_HEK4_20-7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LbCas12a-DR-cr in PBCSK-

   Plasmid

   #126641

   Purpose

   Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

   Plasmid

   #136646

   Purpose

   Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

   Depositor

   Karl Matter, Maria Balda

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
   

   Use

   Tags

   HA (MQDLPGNDNSTAG)

   Expression

   Mammalian

   Mutation

   GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJZC42

   Plasmid

   #66564

   Purpose

   sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1XPP7
   PCP-VP64 IRES mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. REEP5-2Strep

    Plasmid

    #210350

    Purpose

    Expresses REEP5 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    REEP5 (REEP5 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAL190

    Plasmid

    #22279

    Purpose

    Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-CRY2-mCherry

    Plasmid

    #178525

    Purpose

    Expresses CRY2-mCherry in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CRY2-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

    Plasmid

    #192350

    Purpose

    L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    AATG_dttLbCas12a-i_TTCG
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R; E925Q

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-HA-hTRAF3 333-C

    Plasmid

    #44035

    Depositor

    Shao-Cong Sun

    Article

    Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

    Insert

    TNF receptor-associated factor 3 (TRAF3 Human)
    

    Use

    Tags

    1xHA

    Expression

    Mammalian

    Mutation

    Contains amino acids 334-568; S393G and K492N

    Promoter

    Available since

    April 4, 2013

    Availability

    Academic Institutions and Nonprofits only

15. GFP-Myo 1e

    Plasmid

    #134905

    Purpose

    Expression of rat Myo 1e (Myr 3) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Stoffler et al J Cell Biol. 1995 May;129(3):819-30. doi

    Insert

    Myo 1e (rat) with 3' UTR (Myo1e Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-GLT1a-V5

    Plasmid

    #162517

    Purpose

    Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1a (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLpA-JNK1

    Plasmid

    #51945

    Purpose

    Adenoviral vector for JNK1 expression

    Depositor

    Jeffery Molkentin

    Article

    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

    Insert

    JNK1 (Mapk8 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

18. GFP-GLT1b-V5

    Plasmid

    #162518

    Purpose

    Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3-Flag-hDcp2

    Plasmid

    #72213

    Purpose

    mammalian expression of Flag-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q170R (please see depositor's comment below)

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIVT/Smarcc1

    Plasmid

    #122145

    Purpose

    For making Smarcc1 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarcc1 (Smarcc1 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. optoPKAW196R

    Plasmid

    #104548

    Purpose

    Expresses Cry2-mCherry-PKA W196R in mammalian cells

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Cry2-mCherry-PKA W196R
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    PKA W196R

    Promoter

    CMV

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDN-D2irTN2AG5kwh

    Plasmid

    #128253

    Purpose

    Mammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    Beta Globin Intron::hTetR::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV D2ir promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LbCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126643

    Purpose

    Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. NHA-Ago1

    Plasmid

    #21535

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago1 (AGO1 Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

25. dTomato_LD 0_CS G_tTA-BFP

    Plasmid

    #58871

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PH-miRFP-FRB

    Plasmid

    #193568

    Purpose

    Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    PH domain from PLC delta1 (Plcd1 Rat)
    

    Use

    Tags

    FRB (T2098L) and miRFP703

    Expression

    Mammalian

    Mutation

    PH domain

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-Fah_W

    Plasmid

    #163087

    Purpose

    expressing mouse wild type FAH in mammalian cells

    Depositor

    Joseph Takahashi

    Article

    Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

    Insert

    Fah_W
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

    Plasmid

    #89872

    Purpose

    expresses CB1R-iTango component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    CB1R-V2tail-TevN-BLITz1-TetR-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

    Plasmid

    #112257

    Purpose

    Expresses RGS component of opto-RGS2 in mammalian cells

    Depositor

    Brian Chow

    Article

    Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

    Insert

    CRY2-mCherry-(GGGGS)3-hRGS2box
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCherry_LD 0_CS G_tTA-BFP

    Plasmid

    #58856

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge