We narrowed to 9,098 results for: Pol;

1. pCAG_SRGAP2A_SH3(W765A)_HA

   Plasmid

   #222614

   Purpose

   Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Point mutation W765A (SH3 domain)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG SRGAP2A_EVH1(P339L F342C)

   Plasmid

   #222613

   Purpose

   Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutations P339L and F342C

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet28b_His_RAD23A-UBA1

   Plasmid

   #201549

   Purpose

   Expresses UBA1 of human RAD23A in E. coli

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Expresses amino acids 160-205 of DNA polymerase i…

   Promoter

   T7

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pet28b-His_RAD23A-UBA2

   Plasmid

   #201550

   Purpose

   Expresses UBA2 of human RAD23A in E. coli

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Expresses amino acids 311-363 of DNA polymerase i…

   Promoter

   T7

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-SopB(K525A)

   Plasmid

   #183659

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K525A

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-SopB(K528A)

   Plasmid

   #183660

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K528A

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-SopB(33-554)

   Plasmid

   #183661

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 33-554

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-SopB(33-520)

   Plasmid

   #183662

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 33-520

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-SopB(68-554)

   Plasmid

   #183663

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 68-554

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pESC-LEU-IpgD(C439S)

    Plasmid

    #183673

    Purpose

    Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C439S

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. SopB(C460S)-EGFP

    Plasmid

    #183656

    Purpose

    C-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C460S

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EGFP-SopB(C460S)

    Plasmid

    #183658

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C460S

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR207-MUM2sp-Citrine-ADPG2

    Plasmid

    #170725

    Purpose

    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

    Depositor

    George Haughn

    Article

    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

    Insert

    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    

    Use

    Gateway donor vector / entry clone

    Tags

    Citrine tag (714bp)

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR207-MUM2sp-ADPG2

    Plasmid

    #170723

    Purpose

    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

    Depositor

    George Haughn

    Article

    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

    Insert

    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    

    Use

    Gateway donor vector / entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDTCendoNA2

    Plasmid

    #174732

    Purpose

    Expresses DT386C-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a caspase-3/7-cleavable linker

    Depositor

    Jukka Finne

    Article

    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

    Insert

    DT386C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted amino acids 412-560 (the receptor binding…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. deltaVP1 + VP2C-MIOX

    Plasmid

    #166680

    Purpose

    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD

    Plasmid

    #235576

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylation

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Dnmt3a-Dnmt3L (Dnmt3a, Dnmt3l Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208048

    Purpose

    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    BirAopt
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BirAopt is human-codon-optimized BirA (PMID 15707…

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AF20-double-tag

    Plasmid

    #108389

    Purpose

    Express human PC1

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-eGFP-PC1-3HA

    Plasmid

    #108406

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    3HA and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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