We narrowed to 6,278 results for: Cre expression vector

1. pFA6a-ymYPET-CaURA3

   Plasmid

   #118460

   Purpose

   Yeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   YPET A206K, F208S, E232L, N235D

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDZ585 pKAN 2x-mCherry

   Plasmid

   #72236

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   2x-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-ytdTomato-URA3

   Plasmid

   #168054

   Purpose

   Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ytdTomato
   

   Use

   Tags

   ytdTomato

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-link-ymNeongreen-URA3

   Plasmid

   #168059

   Purpose

   Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeonGreen
   

   Use

   Tags

   ymNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-ymScarletI-URA3

   Plasmid

   #168055

   Purpose

   Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymScarletI
   

   Use

   Tags

   ymScarletI

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-ymTq2-URA3

   Plasmid

   #168057

   Purpose

   Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2
   

   Use

   Tags

   ymTurquoise2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-link-ymYPET-URA3

   Plasmid

   #168056

   Purpose

   Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Use

   Tags

   ymYPET

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pR6Kg-Alpha1

   Plasmid

   #165862

   Purpose

   Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-link-ymVenus-URA3

   Plasmid

   #168058

   Purpose

   Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymVenus
   

   Use

   Tags

   ymVenus

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. anti-FLAG M2 light chain

    Plasmid

    #175358

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 light chain

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 light chain
    

    Use

    Tags

    N-terminal secretion signal peptide (MEAPAQLLFLLL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Worm

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

    Plasmid

    #190794

    Purpose

    Template vector to amplify pieces for creating multiplex sgRNA-arrays.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA-scaffold only
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23117

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pColE1-AlphaOmega

    Plasmid

    #165867

    Purpose

    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

    Plasmid

    #192147

    Purpose

    T cell CRISPR lentiviral vector

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-DIO-GRAB_ACh3.0

    Plasmid

    #121923

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

    Plasmid

    #178707

    Purpose

    AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    Dreadd-hM3Dq-HA-P2A-tBFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-PGK-DIO-mCherry-nega miR

    Plasmid

    #137732

    Purpose

    Lentivirus vector for control miR in a Cre-dependent manner

    Depositor

    Tomoyuki Furuyashiki

    Article

    Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

    Insert

    control microRNA
    

    Use

    Lentiviral

    Tags

    mChherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

    Plasmid

    #229826

    Purpose

    Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet-I
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. HR700_HA2TP53_Ex5

    Plasmid

    #228860

    Purpose

    Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5

    Depositor

    Thorsten Stiewe

    Article

    Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. HR700_HA2TP53_Ex6

    Plasmid

    #228861

    Purpose

    Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6

    Depositor

    Thorsten Stiewe

    Article

    Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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