Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,131 results for: LEA

Clear keywords
Showing: 2561 - 2580 of 12131 results

  6. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70703-plasmid-map-sequence-id-127765

    Enlarge

  9. SETD1A

    Plasmid
    #32868
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD1A (SETD1A Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. TBC1D7

    Plasmid
    #32047
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TBC1D7 (TBC1D7 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pPB-C6F/TdTomato

    Plasmid
    #140826
    Purpose
    OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)
    Depositor
    Hideyuki Okano
    Article
    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    C-MYC (MYC Human)
    KLF2 (KLF2 Human)
    NANOG (NANOG Human)
    tdTomato
    Expression
    Mammalian
    Mutation
    L104P- please see depositor comments
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    140826-plasmid-map-sequence-id-307041

    Enlarge

  12. pGBW-m4046955

    Plasmid
    #145616
    Purpose
    Bacterial Expression plasmid for RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145616-plasmid-map-sequence-id-307396

    Enlarge

  16. pcDNA3-ER-CaNAR2

    Plasmid
    #64732
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNAR2
    Tags
    Cerulean3, N-terminal 27 amino acids from CYP450,…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64732-plasmid-map-sequence-id-109351

    Enlarge

Showing: 2561 - 2580 of 12131 results