We narrowed to 7,296 results for: ef1a

1. pEF HA-TSAd-CNS

   Plasmid

   #84558

   Purpose

   Mammalian expression of TSAd-CNS

   Depositor

   Anne Spurkland

   Article

   Granum et al BMC Immunol. 2006 Jul 13;7:15.

   Insert

   TSAd (SH2D2A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deletion of exon 7

   Promoter

   EF1a

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pMCh-1479

   Plasmid

   #82425

   Purpose

   Plasmid for expression of GST-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   nvGW182 NED
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   deleted amino acids 1159-1698 from nvGW182

   Promoter

   EF1A

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCh-1480

   Plasmid

   #82426

   Purpose

   Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   nvGW182 CED
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   amino acids 1-1158 of nvGW182 were deleted

   Promoter

   EF1A

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-BAF

   Plasmid

   #101772

   Purpose

   Expresses EGFP tagged BAF in human cells

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p23-puro-EGFP-RPA194

   Plasmid

   #179237

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   RPA194 (POLR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-Guide-Puro

   Plasmid

   #86708

   Purpose

   Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

   Depositor

   Christoph Bock

   Article

   Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

   Insert

   EF1a-Puro-WPRE-hU6-gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCTIP2-N106

   Plasmid

   #66808

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

   Depositor

   Andrew Yoo

   Article

   Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

   Insert

   CTIP2 isoform b (Bcl11b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AKT-translocation-mStrawberry reporter

   Plasmid

   #158685

   Purpose

   This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-SF3B1-K700E-reporter

    Plasmid

    #200318

    Purpose

    A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Insert

    BSD-2A-EGFPi
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-NPC1(WT)-FLAG

    Plasmid

    #164972

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_3XHA-ATP6V1B2

    Plasmid

    #228864

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCB306-GFP-Rab8A

    Plasmid

    #198470

    Purpose

    lentiviral expression of GFP-Rab8A

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    GFP-Rab8A (RAB8A Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT3-N90-beta-catenin

    Plasmid

    #31785

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-90- Constitutitvely Active

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas-hHDAC3-R265P

    Plasmid

    #103786

    Purpose

    Expresses dCas9 fused to human HDAC3 with R265P mutation

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCas9-HDAC3-R265P (HDAC3 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed arginine 265 to proline

    Promoter

    EF1A

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_123

    Plasmid

    #96919

    Purpose

    for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    p300

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-NPC1(P202A/F203A)-FLAG

    Plasmid

    #164976

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P202A/ F203A

    Promoter

    EF1a

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF5B-FRT-GFP-αTAT1

    Plasmid

    #27099

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
    

    Use

    Tags

    GFP, S-tag, and TEV site

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV_3xFLAG-ATP6V0A3

    Plasmid

    #228866

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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