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pPB_TRE3G_scFV-sfGFP-G9aCD

Plasmid

#235580

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector G9a fused with scFV to programme H3K9me2/3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

G9a (Ehmt2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Dot1LCD

Plasmid

#235582

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dot1l fused with scFV to programme H3K79me2

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Dot1l (Dot1l Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244099

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-tadDE-PcrA M6-UGI

Plasmid

#221236

Purpose

Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

tadDE-PcrA M6-UGI

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244072

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

Plasmid

#61590

Purpose

Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

Depositor

David Komander

Article

Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

Insert

TRABID (ZRANB1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-244 and aa 698-708.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-Ns3h-UGI-T2A-mTagBFP2

Plasmid

#221223

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-Ns3h-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTP261

Plasmid

#104166

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

1x Cysteine located at bps 493-495 in full sequen…

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe puro-eIF4E

Plasmid

#33252

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

eIF4E (EIF4E Human)

Use

Retroviral

Tags

untagged

Expression

Mammalian

Mutation

unmodified

Promoter

SV40

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-2A-tomato SOX2-sgRNA

Plasmid

#196190

Purpose

Editing human SOX2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

SOX2 (SOX2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.H348H.HaloTag

Plasmid

#244070

Purpose

Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Use

AAV

Tags

HaloTag

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244103

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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EGFR G719S

Plasmid

#11013

Purpose

Retroviral construct for expressing human EGFR with G719S mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR G719S (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

G719S

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_scFV-sfGFP-Ezh2FL-CatMut

Plasmid

#235584

Purpose

Dox-inducible expression of control catalytic-mutant Ezh2 fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ezh2 (Ezh2 Mouse)

Use

CRISPR

Mutation

Y726D

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro G3BP1-sgRNA

Plasmid

#196197

Purpose

Cas9 + gRNA expression to edit G3BP1 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

G3BP1 (G3BP1 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D837A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
EGFP-BAF_L58R

Plasmid

#101776

Purpose

Expresses EGFP tagged mutant BAF (L58R) in human cells

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

L58R mutation

Promoter

EF1a

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGM117

Plasmid

#220168

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

CISD1 (CISD1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTargetF-FRT

Plasmid

#113637

Purpose

Derivative of pTargetF with gRNA targeting FRT site

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

sgRNA-FRT

Use

CRISPR

Promoter

pJ23119

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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ATG-1929

Plasmid

#108712

Purpose

CBR2opt in pF4Ag expression vector with T7 and CMV promoter

Depositor

Keith Wood

Article

Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

Insert

CBR2opt

Expression

Bacterial and Mammalian

Mutation

Promega used directed evolution to create CBR (Cl…

Promoter

T7, CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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