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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,839 results for: pcr

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Showing: 2561 - 2580 of 4839 results

  1. GPR174-Tango

    Plasmid
    #66339
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR174 (GPR174 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. RXFP4-Tango

    Plasmid
    #66495
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RXFP4 (RXFP4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pNR1D2-donor

    Plasmid
    #112314
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR1D2 with GFP
    Depositor
    Kevin White
    Insert
    NR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
    Use
    CRISPR
    Mutation
    hg38:3:23977483:A>T, hg38:3:23977515:A>G, h…
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112314-plasmid-map-sequence-id-228473

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  14. YFP-CAAX

    Plasmid
    #223558
    Purpose
    Plasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation box
    Depositor
    Mikel Garcia-Marcos
    Publication
    Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(Venus) - KRas4b
    Expression
    Mammalian
    Available since
    March 28, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    223558-plasmid-map-sequence-id-443640

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  15. ADORA2A-DuET

    Plasmid
    #213174
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADORA2A (ADORA2A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213174-plasmid-map-sequence-id-430742

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  17. pATF1-donor

    Plasmid
    #112328
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF1 with GFP
    Depositor
    Kevin White
    Insert
    ATF1 homology arms flanking EGFP-IRES-Neo cassette (ATF1 Human)
    Use
    CRISPR
    Mutation
    hg38:12:50819876:T>A, hg38:12:50819888:T>C,…
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112328-plasmid-map-sequence-id-228551

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Showing: 2561 - 2580 of 4839 results