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We narrowed to 883 results for: nanoluc

Showing: 601 - 650 of 883 results

  1. pcDNA3.1.Gαi3-LgB91

    Plasmid
    #134359
    Purpose
    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi3-LgB91 (GNAI3 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134359-plasmid-map-sequence-id-306392

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  2. pcDNA3.1.Gαi2-LgB91

    Plasmid
    #134358
    Purpose
    Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi2-LgB91 (GNAI2 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134358-plasmid-map-sequence-id-306397

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  3. pcDNA3.1.Gα12-LgB115

    Plasmid
    #134363
    Purpose
    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα12-LgB115 (GNA12 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134363-plasmid-map-sequence-id-306384

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  4. pcDNA3.1.Gα11-LgB97

    Plasmid
    #134361
    Purpose
    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα11-LgB97 (GNA11 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134361-plasmid-map-sequence-id-306399

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  8. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags
    Depositor
    Veit Hornung
    Available Since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  35. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid
    #207805
    Purpose
    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.
    Depositor
    Koen Breyne
    Article
    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do
    Insert
    E-NoMi
    Use
    Lentiviral
    Tags
    3xFlag tag, copGFP, mCherry, and nanoluciferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207805-plasmid-map-sequence-id-419158

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  36. pSNRW-Z3-Nluc

    Plasmid
    #174486
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z3-Nluc
    Use
    Tags
    6x Histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available Since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174486-plasmid-map-sequence-id-345289

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  37. pSNRW-Z5-Nluc

    Plasmid
    #174487
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z5-Nluc
    Use
    Tags
    6x Histidine tag (N terminal on insert)
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available Since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174487-plasmid-map-sequence-id-345291

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  38. L2111-FKBP-GPA-20-11S

    Plasmid
    #222882
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222882-plasmid-map-sequence-id-444498

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  39. L2375-FKBP-GPA-20-11S

    Plasmid
    #222897
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222897-plasmid-map-sequence-id-441787

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  40. L2376-FKBP-GPA-20-114

    Plasmid
    #222896
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222896-plasmid-map-sequence-id-441634

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  41. L2374-FRB-GPA-20-11S

    Plasmid
    #222895
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222895-plasmid-map-sequence-id-440186

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  42. L2373-FRB-GPA-20-114

    Plasmid
    #222894
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222894-plasmid-map-sequence-id-440207

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  43. L2110-FKBP-GPA-20-114

    Plasmid
    #222881
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222881-plasmid-map-sequence-id-440211

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  44. L2108-FRB-GPA-20-114

    Plasmid
    #222879
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222879-plasmid-map-sequence-id-440199

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  45. L2109-FRB-GPA-20-11S

    Plasmid
    #222880
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222880-plasmid-map-sequence-id-440181

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  46. L2096-GPA-BRET-Biosensor-Retrovirus-TD146

    Plasmid
    #222878
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222878-plasmid-map-sequence-id-446610

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  47. L2379-FKBP-GPA(V84R)-20-11S

    Plasmid
    #222901
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA(V84R)-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222901-plasmid-map-sequence-id-441651

    Enlarge

  48. L2380-FKBP-GPA(V84R)-20-114

    Plasmid
    #222900
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA(V84R)-20GS-NanoLuc114
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222900-plasmid-map-sequence-id-441786

    Enlarge

  49. L2378-FRB-GPA(V84R)-20-11S

    Plasmid
    #222899
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222899-plasmid-map-sequence-id-440196

    Enlarge

  50. L2377-FRB-GPA(V84R)-20-114

    Plasmid
    #222898
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-20GS-NanoLuc114
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222898-plasmid-map-sequence-id-440197

    Enlarge

Showing: 601 - 650 of 883 results