We narrowed to 594 results for: pFAST

1. pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)

   Plasmid

   #30123

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)

   Plasmid

   #223766

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   EGFP-TEV-GST

   Expression

   Insect

   Mutation

   W18A/L21A; deletion of LIR

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346

   Plasmid

   #217012

   Purpose

   Expression of CDK7 in insect cells

   Depositor

   Manjeet Mukherjee

   Article

   Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

   Insert

   CDK7 (S164D, T170E)_del327-346 (CDK7 Human)
   

   Use

   Tags

   TEV cleavable his tag

   Expression

   Insect

   Mutation

   (S164D, T170E)_del327-346

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

   Plasmid

   #48295

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

   Plasmid

   #55219

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

   Plasmid

   #48296

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   His6 and MBP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)

   Plasmid

   #223777

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Mutation

   E44A L47A D49A A50K Q51A; disrupts WIPI2 binding

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

   Plasmid

   #55220

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   His6 and MBP, Asn10

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

   Plasmid

   #55217

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

    Plasmid

    #55216

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

    Plasmid

    #48297

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    StrepII and msfGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

    Plasmid

    #55221

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    StrepII and msfGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

    Plasmid

    #55222

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

    Plasmid

    #55223

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEND-193_Cacnes_HS

    Plasmid

    #225622

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEND-192_Cacnes_HS

    Plasmid

    #225621

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Golgi-FAST-IRES-iRFP670

    Plasmid

    #216345

    Purpose

    For testing localization of pFAST and iRFP670 proteins to Golgi complex

    Depositor

    Alison Tebo

    Insert

    ST6Gal1_pFAST-IRES-Sys1_iRFP670
    

    Use

    Tags

    myc for FAST and iRF670 proteins

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Peroxisomes-FAST-IRES-iRFP670

    Plasmid

    #216347

    Purpose

    For testing localization of pFAST and iRFP670 proteins to peroxisomes

    Depositor

    Alison Tebo

    Insert

    pFAST_SKL-IRES-iRFP670_PXMP2
    

    Use

    Tags

    myc for FAST protein only

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Mito-FAST-IRES-iRFP670

    Plasmid

    #216346

    Purpose

    For testing localization of pFAST and iRFP670 proteins to mitochondria

    Depositor

    Alison Tebo

    Insert

    Cox8_pFAST-IRES-Tomm20_iRFP670
    

    Use

    Tags

    myc for FAST and iRF670 proteins

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ER-FAST-IRES-iRFP670

    Plasmid

    #216344

    Purpose

    For testing localization of pFAST and iRFP670 proteins to endoplasmic reticulum

    Depositor

    Alison Tebo

    Insert

    PRL_pFAST-IRES-CANX_iRFP670
    

    Use

    Tags

    myc for FAST and iRF670 proteins

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge