We narrowed to 1,240 results for: pFAST

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pFastBac1-LRRK2 WD40

Plasmid

#139211

Purpose

Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

Depositor

Hao Wu

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

Insert

LRRK2 (LRRK2 Human)

Tags

N-His and MBP

Expression

Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with LFn + Fla

Plasmid

#84866

Purpose

Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Use

Baculovirus

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pAG657 H2B-pFAST

Plasmid

#172862

Purpose

Expresses pFAST fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

H2B-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC8 H143A

Plasmid

#224344

Purpose

Expresses human KDAC8 (HDAC8) H143A in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 143 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC8 D101E

Plasmid

#224350

Purpose

Expresses human KDAC8 (HDAC8) D101E in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Aspartate 101 mutated to glutamate

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac HTB DPP9L

Plasmid

#166788

Purpose

Insect cell expression vector for DPP9L (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9S

Plasmid

#166787

Purpose

Insect cell expression vector for DPP9S (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac flag Bmi1

Plasmid

#1967

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Bmi1 (Bmi1 Mouse)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac1_GI.1_Q62C-A140C

Plasmid

#162581

Purpose

Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Glutamine 62 changed to Cysteine and Alanine 140 …

Promoter

polyhedring

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-His6-Mps1-WT

Plasmid

#114063

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-WT (TTK Human)

Tags

His6

Expression

Insect

Mutation

none

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac GST-SAS6

Plasmid

#80273

Purpose

baculovirus creation plasmid for GST-SAS6

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

SAS6 (SASS6 Human)

Tags

GST

Expression

Insect

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac K-Flag Ring1A

Plasmid

#1995

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Ring1A (Ring1 Mouse)

Tags

flag

Expression

Insect

Mutation

Kozak before start codon

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pAG665 MAP4-pFAST

Plasmid

#172865

Purpose

Expresses MAP4-pFAST in mammalian cells (microtubule labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

MAP4-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac GST-Plk4

Plasmid

#80264

Purpose

baculovirus creation plasmid for GST-Plk4

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

GST

Expression

Insect

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with LFn + FlaAAA

Plasmid

#84867

Purpose

Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
FlaAAA

Use

Baculovirus

Tags

6xHis

Expression

Insect

Mutation

3 C-terminal leucines have been replaced by alani…

Promoter

Polyhedrin

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac mph1 flag

Plasmid

#1985

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

mPh1 (Phc1 Mouse)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac flag Mel18

Plasmid

#1968

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Mel18 (Pcgf2 Mouse)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac(His10-Shp2)

Plasmid

#177899

Purpose

Baculo expression of His10 tag fused to human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Tags

10xHis-TEV-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pfastbac/mBmal62-447

Plasmid

#47335

Purpose

mBmal fragment cloned into pfastbac and crystallized

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Mutation

aa 62-447

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pfastbacHTb/mClock26-384

Plasmid

#47336

Purpose

mClock fragment cloned into pfastbacHTb vector with Hexa His tag

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

His and Tev

Mutation

aa 26-384

Promoter

PH

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only