We narrowed to 13,194 results for: lic

1. pMMK ENTR 2 hU6 ACTB sgRNA

   Plasmid

   #206270

   Purpose

   ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   ACTB sgRNA
   

   Use

   CRISPR; Multimate/gateway entr 2

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMMK ENTR 2 hU6 HEK3 PegRNA

   Plasmid

   #206277

   Purpose

   ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   HEK3 PegRNA
   

   Use

   CRISPR; Multimate/gateway entr 2

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMMK ENTR 3 polH aeBlue polH VSV-G

   Plasmid

   #206278

   Purpose

   ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   aeBlue, VSV-G
   

   Use

   Multimate/gateway entr 3

   Expression

   Bacterial and Insect

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.0-TERT_-100_-79

   Plasmid

   #193785

   Purpose

   luciferase reporter for duplicated TERT promoter

   Depositor

   Joseph Costello

   Article

   Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

   Insert

   TERT promoter (TERT Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   TERT

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDA2ScIfm

   Plasmid

   #193786

   Purpose

   Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Frame-shifted CI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLTATAA synthetic promoter carrying binding sites…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLA2ScIfmTcIfm

   Plasmid

   #193792

   Purpose

   Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLLacO-1 and PLTetO-1

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. deltaVP1d63C + VP2C-GFPdeg

   Plasmid

   #182481

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-GFPdeg
   deltaVP1d63C
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Linked VLP NES

   Plasmid

   #182432

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS-NES
   deltaVP1
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Linked VLP NES (M)

   Plasmid

   #182434

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(M)-NES
   deltaVP1
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Linked VLP NES (G)

    Plasmid

    #182476

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(G)-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

    Plasmid

    #182918

    Purpose

    Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

    Depositor

    Daniel Gerlich

    Article

    Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

    Insert

    pH2B (H2BC16P Human)
    

    Tags

    mNeonGreen-FRB(DmrC)

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-D-kcnj1b

    Plasmid

    #173126

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj1b (kcnj1b Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-kcnj8

    Plasmid

    #173134

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj8 (kcnj8, si:dkey-183c2.2 Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPICZ-pre-Ost1-pro-alphaf-Btl2

    Plasmid

    #117665

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-pro-alphaf-Btl2
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. exon K-renilla

    Plasmid

    #81007

    Purpose

    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

    Depositor

    Richard Baines

    Article

    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

    Insert

    paralytic (para Fly)
    

    Tags

    Renilla luciferase

    Expression

    Insect

    Mutation

    spanning exon 24 to exon 26, termination codon in…

    Promoter

    P-AC5 (Actin 5C)

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIG1_UL136_26kDa_Myc

    Plasmid

    #74955

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)

    Expression

    Mammalian

    Mutation

    delta (1-62) M78A M80A M100A M128A

    Promoter

    CMV, Lentiviral LTR

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIG1_UL136_33kda_Myc

    Plasmid

    #74957

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    Myc tag (EQKLISEEDL)

    Expression

    Mammalian

    Mutation

    M63A M78A M80A M100A M128A

    Promoter

    CMV, Lentiviral LTR

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2 mir-1-1 reporter mir16-1.3

    Plasmid

    #46682

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.3 (MIR16-1 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.2 mir-1-1 reporter mir16-1.1

    Plasmid

    #46680

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.1 (MIR16-1 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCin4-3xFLAG-MKL1 L555A

    Plasmid

    #19851

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 L555A (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Leucine 555 to Alanine

    Available Since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only