We narrowed to 11,100 results for: CHL

1. pET151-hUCH37 DM

   Plasmid

   #61931

   Purpose

   Expression of hUCH37 (ISF1) M269E/N291D in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) M269E/N291D (UCHL5 Human)
   

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Changed methionine 269 to glutamate and asparagin…

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET151-hUCH37 (ISF1)(M148A/F149A)

   Plasmid

   #61933

   Purpose

   Expression of hUCH37 (M148A/F149A) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) (UCHL5 Human)
   

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Methionine 148 and Phenylalanine 149 were mutated…

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pFL-TK-AID3'UTR-DEL

   Plasmid

   #19988

   Depositor

   Nina Papavasiliou

   Article

   Teng et al Immunity. 2008 May . 28(5):621-9.

   Insert

   AID 3' UTR (Aicda Mouse)
   

   Expression

   Mammalian

   Mutation

   Deletion

   Available Since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC015 - dLwCas13a-NF

   Plasmid

   #91905

   Purpose

   Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

   Expression

   Mammalian

   Mutation

   R474A and R1046A

   Promoter

   CAG

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV sgRNA-Notch1 #1 GFP

   Plasmid

   #106950

   Purpose

   Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   gRNA targeting Notch1 (Notch1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIP-CMV-tagRFP-FLAG-cGAS

   Plasmid

   #86676

   Purpose

   Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   tagRFP-FLAG-cGAS
   

   Use

   Lentiviral

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH7WG2B-OsMIR390-B/c

   Plasmid

   #61465

   Purpose

   Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

   Depositor

   James Carrington

   Article

   Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

   Insert

   OsMIR390-B/c
   

   Expression

   Plant

   Mutation

   O. sativa MIR390 precursor sequence including a c…

   Promoter

   OsUBI

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Thomas Westbrook, Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

   Plasmid

   #35511

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Promoter

    TgTUB1

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEND-349_Cacnes_ori

    Plasmid

    #225604

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMZ828

    Plasmid

    #155322

    Purpose

    Constitutive expression of PhyB(1-650)-VP16 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p206-Switch-ON

    Plasmid

    #217885

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn_CapChR2_mScarlet

    Plasmid

    #188039

    Purpose

    Expresses CapChR2-mScarlet in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Use

    AAV

    Tags

    mScarlet

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    hSyn

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. U6-sgRNA-GFP

    Plasmid

    #200068

    Purpose

    For the knock down of GFP with an astrocyte specific mCherry expression

    Depositor

    Baljit Khakh

    Article

    Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

    Insert

    U6-sgRNA-GFP
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    GfaABC1D-mCherry

    Promoter

    U6, GfaABC1D

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIP-SFFV-EGFP-NLS

    Plasmid

    #86677

    Purpose

    Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    EGFP-NLS
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIP-CMV-GFP-FLAG-cGAS

    Plasmid

    #86675

    Purpose

    Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Syn_CapChR2_eYFP

    Plasmid

    #188035

    Purpose

    Expresses CapChR2-eYFP in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    hSyn

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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