We narrowed to 152,655 results for: des

1. pyc111m

   Plasmid

   #158779

   Purpose

   ribosome-tagged, Cre-dependent GCaMP for soma localization

   Depositor

   Jennifer Garrison, Zachary Knight

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   GCaMP6m-RPL10a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO_EGFP-RandE3

   Plasmid

   #79872

   Purpose

   For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. G1397 DddAtox-C–SaKKH-Cas9(D10A)

   Plasmid

   #157840

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

   Plasmid

   #195049

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pPGI

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJET1.2-U6

   Plasmid

   #173157

   Purpose

   Single guide RNA cassette under U6 promoter

   Depositor

   Piotr Ziolkowski

   Article

   Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

   Insert

   sgRNA cassette under U6 promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ND4 left TALE–G1333 DddAtox C

   Plasmid

   #158092

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ND4 right TALE–G1333 DddAtox N

   Plasmid

   #158096

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. G1333 DddAtox-C–SaKKH-Cas9(D10A)

   Plasmid

   #157838

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. G1397 DddAtox-N–SaKKH-Cas9(D10A)

   Plasmid

   #157839

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. (K) tNOS (S)_T3 (SBE120)

    Plasmid

    #195010

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (K) tNOS (S)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. (H) tNOS (I) _T2 (SBE119)

    Plasmid

    #195006

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (H) tNOS (I)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. (B) HYG marker(R) (SBE264)

    Plasmid

    #195013

    Purpose

    hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+HYG+tGPD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. (E) tNOS (F) _T1 (SBE118)

    Plasmid

    #194997

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (E) tNOS (F)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. (B) BLE marker(R) (SBE262)

    Plasmid

    #195012

    Purpose

    bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+BLE+tGPD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pyc128m

    Plasmid

    #158778

    Purpose

    soluble GCaMP expression

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pORANGE Cacnb4-GFP KI

    Plasmid

    #139663

    Purpose

    Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORANGE GFP-Cacnb1 KI

    Plasmid

    #139660

    Purpose

    Endogenous tagging of CaVβ1: N-terminal (amino acid position: S8)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. (R)pGPD1(D)_P1(SBE109)

    Plasmid

    #194993

    Purpose

    promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (R) pGPD (D)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. (F)pGPD(G)_P2 (SBE110)

    Plasmid

    #194992

    Purpose

    promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (F) pGPD (G)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. (I) pADH2 (J) P3 (SBE114)

    Plasmid

    #187629

    Purpose

    promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (I) pADH2 (J)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge