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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2601 - 2620 of 5523 results

  2. DVC_p15A_AE

    Plasmid
    #217587
    Purpose
    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Publication
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    217587-plasmid-map-sequence-id-468600

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  3. pSBLego

    Plasmid
    #219967
    Purpose
    The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.
    Depositor
    Markus Seeger
    Publication
    Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10
    Type
    Empty backbone
    Tags
    SLEHHHHHH and pelB signal sequence
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    219967-plasmid-map-sequence-id-435554

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  15. pMVRha

    Plasmid
    #229553
    Purpose
    Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.
    Depositor
    Sandy Schmidt
    Publication
    Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229553-plasmid-map-sequence-id-461178

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  16. pHW1385

    Plasmid
    #229879
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Depositor
    Han Wang
    Publication
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Tags
    mScarlet-I-C1
    Expression
    Worm
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229879-plasmid-map-sequence-id-460859

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  18. PAC_E83_LOV_eGFP

    Plasmid
    #231574
    Purpose
    This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light
    Depositor
    Dominik Niopek
    Publication
    Wolf et al Nat Methods. 2025 Aug;22(8):1698-1706. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
    Expression
    Mammalian
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    231574-plasmid-map-sequence-id-460758

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  20. pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227892
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EEA1 homology arms with mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227892-plasmid-map-sequence-id-455731

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Showing: 2601 - 2620 of 5523 results