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pAV0761

Plasmid

#133494

Purpose

Expression of nucleo-cytoplasmic mTagBFP2 in P-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pADtet-His6-MscL

Plasmid

#83373

Purpose

Expresses wild type MscL in mammalian cells

Depositor

Allen Liu

Article

Heureaux et al Cell Mol Bioeng. 2014 Sep;7(3):307-319.

Insert

Mechanosensitive channel of large conductance (mscL E. coli)

Use

Adenoviral

Tags

His6 tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only
pTC201B

Plasmid

#52193

Purpose

for creating reversible targeting construct

Depositor

Tian Chi

Article

Chaiyachati et al BMC Biol. 2012 Nov 30;10:96. doi: 10.118

Insert

promoter-trap cassette Neo-Ires-GFP

Use

Cre/Lox

Tags

Neo, GFP

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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YEp112-CUP1-His-Smt3

Plasmid

#99537

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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YEp195-CUP1-His-Smt3

Plasmid

#99538

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAV0327

Plasmid

#133488

Purpose

Expression of nucleo-cytoplasmic sfGFP

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0478

Plasmid

#133498

Purpose

Expression of sfGFP targeted to the nucleus with two NLS signals

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTR1-GPD-TRPC

Plasmid

#190103

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection

Depositor

Perng-Kuang Chang

Article

Chang et al Appl Environ Microbiol. 2012 Nov;78(21):

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAV0471

Plasmid

#133490

Purpose

Expression of nucleo-cytoplasmic mTagBFP2

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(tdh1*)-mTagBFP2-terminator(tdh1)

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0710

Plasmid

#133517

Purpose

Expression of mCherry tagged beta-tubulin to monitor microtubules

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(atb2)-mCherry-atb2-terminator(atb2)-hphMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0663

Plasmid

#133478

Purpose

Expression in fission yeast from promoter pact1, integrated at his5 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0770

Plasmid

#133518

Purpose

Expression of mTagBFP2 tagged beta-tubulin to monitor microtubules

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(atb2)-mTagBFP2-atb2-terminator(atb2)-hphMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0624

Plasmid

#133491

Purpose

Expression of cytoplasmic 3mTagBFP2

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(tdh1)-3mTagBFP2-terminator(tdh1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0523

Plasmid

#133495

Purpose

Expression of nucleo-cytoplasmic sfGFP in M-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(mam1*)-sfGFP-terminator(ScADH1)-kanMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0771

Plasmid

#133513

Purpose

Expression of mTagBFP2 tagged CHD domain to monitor actin cytoskeleton

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-3mTagBFP2-CHDRng2-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0479

Plasmid

#133499

Purpose

Expression of sfGFP excluded from the nucleus with two NES signals

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0543

Plasmid

#133493

Purpose

Expression of nucleo-cytoplasmic mCherry in P-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0662

Plasmid

#133477

Purpose

Expression in fission yeast from promoter pact1, integrated at lys3 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCuHA-ATG13[3SA](426)

Plasmid

#122076

Purpose

Expressing HA-ATG13[3SA]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1+2

Plasmid

#17716

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+2 (SUP35 Budding Yeast)

Expression

Yeast

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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