We narrowed to 6,485 results for: plasmid dna

1. pDGB1omega2_SF (GB0466)

   Plasmid

   #68243

   Purpose

   Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Solanum lycopersicum intergenic region
   

   Use

   Synthetic Biology; Unspecified

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB1alpha2_SF (GB0107)

   Plasmid

   #68233

   Purpose

   Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Solanum lycopersicum intergenic region
   

   Use

   Synthetic Biology; Unspecified

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCALNL-eGFP-NT1-6-gix-6-NT1

   Plasmid

   #81204

   Purpose

   contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   gix-Neo-gix deletion cassette upstream of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCBa

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

   Plasmid

   #69225

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS2 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

   Plasmid

   #69226

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS3 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. AP1297‐1

   Plasmid

   #87247

   Purpose

   gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

   Insert

   gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
   

   Use

   Tags

   gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AL08_pgRNA.DMD

   Plasmid

   #100293

   Purpose

   DMD intron 43-targeting gRNA expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 Polymerase III snRNA

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFP121

   Plasmid

   #21670

   Depositor

   James Haber

   Article

   Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

   Insert

   Lacz (lacZ E. coli)
   

   Use

   Centromeric plasmid

   Tags

   Expression

   Mutation

   The second lacZ sequence has been modified, the E…

   Promoter

   Available since

   Sept. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

9. Bxb1-GT_CAG-ITPKA-mScarlet-I

   Plasmid

   #229778

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Tags

   ITPKA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem

    Plasmid

    #229793

    Purpose

    Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGK58

    Plasmid

    #228118

    Purpose

    Express node 814 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 814, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGK65

    Plasmid

    #228123

    Purpose

    Express node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGK59

    Plasmid

    #228119

    Purpose

    Express node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAGK57

    Plasmid

    #228117

    Purpose

    Express node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAGK56

    Plasmid

    #228116

    Purpose

    Express node 412 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 412, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGK60

    Plasmid

    #228120

    Purpose

    Express node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGK55

    Plasmid

    #228114

    Purpose

    Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL NarL YdfI Flag

    Plasmid

    #225650

    Purpose

    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    NarL-YdfI (narL Synthetic)
    

    Use

    Cell free expression

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    NarL with native DNA-binding domain replaced with…

    Promoter

    T7

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL0_EF-Fld

    Plasmid

    #202181

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Fld terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

    Plasmid

    #200639

    Purpose

    Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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