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pILGFP10CF72

Plasmid

#185857

Purpose

Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10E1B

Plasmid

#185856

Purpose

Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10E1A

Plasmid

#185855

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3A2 (1)

Plasmid

#185851

Purpose

Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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KBB441

Plasmid

#185131

Purpose

Swi6 full length with L->A mutated in nuclear localization signal

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

SWI6 deletion of amino acids 450-458

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9341 (pgRNA_IX-1_NatMX)

Plasmid

#161593

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9343 (pgRNA_XV-1_NatMX)

Plasmid

#161595

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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KBB345

Plasmid

#185078

Purpose

rfa3D46 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA3

Tags

GFP

Expression

Yeast

Mutation

Rfa3 truncation aa 1-46

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB419

Plasmid

#185089

Purpose

Swi6L-GFP expressing N-term 273 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation at aa 273

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB354

Plasmid

#185082

Purpose

Swi6L-GFP expressin N-term 273 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation aa 1-273

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB416

Plasmid

#185087

Purpose

Swi6L-GFP with nuclear export signal mutated to alanines

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 nuclear export signal L to A mutations

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB349

Plasmid

#185080

Purpose

rfa2D248 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA2

Tags

GFP

Expression

Yeast

Mutation

Rfa2 truncation aa 1-247

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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KBB353

Plasmid

#185081

Purpose

Swi6M-GFP expressing N-term 181 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation aa 1-181

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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KBB484

Plasmid

#185132

Purpose

SWI6-GFP full length under control of GAL1 promotion

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB486

Plasmid

#185133

Purpose

Full length CRM1 fused to binding domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

CRM1

Expression

Yeast

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB491

Plasmid

#185134

Purpose

Full length SWI6 fused to activation domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Expression

Yeast

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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L1_UBE2:eG-Lt-Ck4

Plasmid

#136130

Purpose

L1 in position 4, mark cell outline with eGFP

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:eGFP-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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PROM_MpNOP1

Plasmid

#136117

Purpose

PROM for cloning with 5UTR_MpNOP1

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_MpNOP1 Mapoly0008s0036

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_MpNOP1

Plasmid

#136119

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_MpNOP1 Mapoly0008s0036

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_NOP1:mS-N7-Ck2

Plasmid

#136126

Purpose

L1 in position 2, example of weak promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpNOP1:mScarletI-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:mT-N7-Ck2

Plasmid

#136127

Purpose

L1 in position 2, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:mT-N7-Ck3

Plasmid

#136128

Purpose

L1 in position 3, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:eG-Lt-Ck3

Plasmid

#136129

Purpose

L1 in position 3, mark cell outline with eGFP

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:eGFP-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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5UTR_MpNOP1

Plasmid

#136103

Purpose

5UTR for cloning with PROM_MpNOP1

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpNOP1 Mapoly0008s0036

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_TP-MpSIG2

Plasmid

#136093

Purpose

Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

TP-MpSIG2, Mapoly0214s0004

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+-wbp2nlY282F

Plasmid

#172468

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY282F

Use

Xenopus expression

Tags

none

Mutation

changed Tyrosine at aa282 to Phenylalanine

Promoter

SP6

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Ecm2 1-197

Plasmid

#169924

Purpose

WT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2 1-197

Expression

Bacterial and Yeast

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF1 (G180A)

Plasmid

#175415

Purpose

Encodes His-MBP-TEV-ybbR-G180A eRF1

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF1 (SUP45 Budding Yeast)

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

G180A

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlF127P

Plasmid

#174507

Purpose

expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlF127P

Use

Xenopus expression

Tags

none

Mutation

changed Phenylalanine at aa127 to Proline

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-wbp2nlT45A

Plasmid

#172464

Purpose

expresses protein with one aa substitution to affect potential phosphorylation

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlT45A

Use

Xenopus expression

Tags

none

Mutation

changed Threonine at aa45 to Alanine

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-wbp2nlY55F

Plasmid

#172466

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY55F

Use

Xenopus expression

Tags

none

Mutation

changed Tyrosine at aa55 to Phenylalanine

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Mdn1-FL

Plasmid

#171602

Purpose

Expresses full length S. pombe Mdn1 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Tags

His6 tag

Expression

Insect

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI763 p(Z)-mCherry + UBC9 sgRNA

Plasmid

#164918

Purpose

CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(Z)-mcherry

Use

CRISPR

Expression

Yeast

Promoter

P(Z), synthetic promoter

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2082

Plasmid

#155162

Purpose

Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaFΔ[248-269])-(6xHis-TEV-MlaB)

Expression

Bacterial

Mutation

deleted amino acids 248-269 in MlaF

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1514

Plasmid

#155147

Purpose

Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1965

Plasmid

#155152

Purpose

Expresses MlaF(1-246)-(6 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1966

Plasmid

#155153

Purpose

Expresses MlaF(1-246)-(10 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1967

Plasmid

#155154

Purpose

Expresses MlaF(1-246)-(14 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_007_026_MV1

Plasmid

#134419

Purpose

pYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_008_026_MV1

Plasmid

#134420

Purpose

pYTKMV1-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pRPL18B

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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