We narrowed to 22,471 results for: ESC

1. pSCMV-H2B-FT-Slow

   Plasmid

   #157671

   Purpose

   Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

   Depositor

   Shangqin Guo

   Article

   Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

   Insert

   Histone H2B
   

   Use

   Retroviral

   Tags

   Slow-FT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AsdCas12a VPR

   Plasmid

   #188512

   Purpose

   Expresses FLAG tagged AsdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LbdCas12a VPR

   Plasmid

   #188513

   Purpose

   Expresses FLAG tagged LbdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD/HisD-PATagRFP

   Plasmid

   #31945

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   araB

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVSVG-PATagRFP

   Plasmid

   #31947

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Use

   Tags

   VSVG

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDUAL_2_araBAD

   Plasmid

   #155176

   Purpose

   see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKRaION1-N1

   Plasmid

   #177801

   Purpose

   Mammalian expression of green fluorescent potassium indicator KRaION1

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   KRaION1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-GCaMP3bright

   Plasmid

   #74423

   Purpose

   Express fluorescent calcium indicator GCaMP3bright in mammalian cells

   Depositor

   Katalin Torok

   Article

   Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

   Insert

   GCaMP3bright
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D392A

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiRFP703-Tubulin

   Plasmid

   #79991

   Purpose

   Alpha tubulin labeling with near-infrared fluorescent protein miRFP703

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   miRFP703

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHLuc Control

    Plasmid

    #141091

    Purpose

    pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-CMV-TetO2_EmGFP

    Plasmid

    #113892

    Purpose

    Mammalian (inducible) expression of cytosolic EmGFP

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    Emerald GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCI-syn-fRGECO2

    Plasmid

    #125244

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W44Y, D432A

    Promoter

    synapsin

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pATLIC

    Plasmid

    #110287

    Purpose

    A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

    Depositor

    In-Geol Choi

    Article

    Ko et al Appl Environ Microbiol. 2012 May;78(9):3

    Insert

    yfaL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRE161

    Plasmid

    #118509

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMito-miRFP718nano

    Plasmid

    #195754

    Purpose

    Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    Human cytochrome C oxidase subunit VIII-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH2B-miRFP718nano

    Plasmid

    #195750

    Purpose

    Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    H2B-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMRE164

    Plasmid

    #118512

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAD-SPS6-Pman

    Plasmid

    #133906

    Purpose

    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(SPS6)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-CMV-TetO2_mTurquoise2

    Plasmid

    #113895

    Purpose

    Mammalian (inducible) expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Turquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-GCaMP3fast

    Plasmid

    #74422

    Purpose

    Express fluorescent calcium indicator GCaMP3fast in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3fast
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W4Y D356A

    Promoter

    CMV

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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