We narrowed to 13,319 results for: SHI

1. pcDNA5-F-Med26-K74A K75A

   Plasmid

   #49253

   Purpose

   Mammalian expression of flag-tagged human Med26-K74A K75A

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med 26 (MED26 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K74A K75A

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pColdI-DDX3_helicase_core_Q360L

   Plasmid

   #233619

   Purpose

   Expression of His-tagged helicase core region of DDX3X with Q360L mutation in E.coli

   Depositor

   Shintaro Iwasaki

   Article

   Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

   Insert

   DDX3X helicase core Gln360Leu (DDX3X Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Gln360Leu

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pColdI-DDX3_helicase_core_Q360P

   Plasmid

   #233620

   Purpose

   Expression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coli

   Depositor

   Shintaro Iwasaki

   Article

   Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

   Insert

   DDX3X helicase core Gln360Pro (DDX3X Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Gln360Pro

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPBpuro-EGFP-eDHFR(69K6)-cRaf

   Plasmid

   #178849

   Purpose

   Mammalian expression of cRaf fused to EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-cRaf (RAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPBpuro-cRaf-mNG-eDHFR(69K6)

   Plasmid

   #172107

   Purpose

   Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   cRaf-mNeonGreen-eDHFR(69K6) (RAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-F-Med26-NTD

   Plasmid

   #49255

   Purpose

   Mammalian expression of flag-tagged Med26-NTD

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med 26 (MED26 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   NTD (aa 1-339)

   Promoter

   Available since

   Dec. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5-F-Med26-R61A K62A

   Plasmid

   #49254

   Purpose

   Mammalian expression of flag-tagged human Med26 R61A K62A

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med26 (MED26 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R61A K62A

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pMXs-hs-EGR1

   Plasmid

   #52724

   Purpose

   retroviral expression of human EGR1

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   EGR1 (EGR1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

   Plasmid

   #134925

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid

    #134922

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid

    #134924

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid

    #134923

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs-hs-KRAS

    Plasmid

    #52729

    Purpose

    retroviral expression of human KRAS

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

    Plasmid

    #178854

    Purpose

    Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Grb2: Y160E

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

    Plasmid

    #172105

    Purpose

    Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    miRFP670-eDHFR(69K6)-Gαs (GNAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Gαs: C3S

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-F-Med26-NTD R61A K62A

    Plasmid

    #49256

    Purpose

    Mammalian expression of flag-tagged human Med26-NTD R61A K62A

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    NTD R61A K62A

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5-F-Med26-NTD- K74A K75A

    Plasmid

    #49252

    Purpose

    Mammalian expression of flag-tagged human Med26-NTD- K74A K75A

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K74A, K75A, aa 1-339

    Promoter

    Available since

    Dec. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCAGGS-FuG-E (P440E)

    Plasmid

    #119978

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E (P440E) envelope coating virus particle with NeuRet.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci Methods. 2018 Oct 19;311:147-

    Insert

    Fusion Glycoprotein type E (P440E)
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-PGK-DIO-mCherry-nega miR

    Plasmid

    #137732

    Purpose

    Lentivirus vector for control miR in a Cre-dependent manner

    Depositor

    Tomoyuki Furuyashiki

    Article

    Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

    Insert

    control microRNA
    

    Use

    Lentiviral

    Tags

    mChherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYO1025

    Plasmid

    #235705

    Purpose

    Expression of FL human VPS34 and human VPS15-ZZ in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Inserts

    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    

    Use

    Tags

    3xTEV-ZZ

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and CMV

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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