We narrowed to 152,655 results for: des

1. pKLV-EF1amCherry-W

   Plasmid

   #159295

   Purpose

   Lentiviral vector expressing mCherry driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PLKO-mcherry-luc-puro-Renilla cDNA

   Plasmid

   #29783

   Depositor

   Carl Novina

   Article

   Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

   Insert

   Renilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR223-EBNA2

   Plasmid

   #195815

   Purpose

   Gateway Entry Clone of Epstein-Barr virus EBNA2

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   EBNA2 (EBNA-2 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-FLAG-HA-CAD

   Plasmid

   #46238

   Purpose

   For expression of HA and FLAG-tagged murine CAD in mammalian cells

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_CaMKIIa_SERT_EYFP

   Plasmid

   #198737

   Purpose

   Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

   Depositor

   Lin Tian

   Article

   Vargas et al Science. 2023 Feb 17;379(6633):700-706.

   Insert

   Serotonin transporter and yellow fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

   Plasmid

   #109320

   Purpose

   Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

   Depositor

   William Lagor

   Article

   Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1_AJMA

   Plasmid

   #183760

   Purpose

   Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Inserts

   ASAP2f
   jRCaMP1b
   miRFP703
   ASAP2f
   

   Use

   Tags

   Lck and NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR223-EBNA3C

   Plasmid

   #198576

   Purpose

   Gateway Entry Clone

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   EBNA3C (EBNA-3B/EBNA-3C Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   None (wildtype)

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCSF107mT-GATEWAY-3'-FLAG

   Plasmid

   #67619

   Purpose

   A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

   Depositor

   Todd Stukenberg

   Article

   Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

   Type

   Empty backbone

   Use

   In vitro transcription or translation off of an s…

   Tags

   C-terminal Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP gRNA (BRDN0000562805)

    Plasmid

    #80035

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pD3sE_SC12

    Plasmid

    #219720

    Purpose

    DV3 E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV3 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR223-EBNA3B

    Plasmid

    #198574

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA3B (EBNA-3B/EBNA-3C Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR223-EBNA3A

    Plasmid

    #198575

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA3A (EBNA-3A Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLYS1-MICU1-Flag

    Plasmid

    #50058

    Purpose

    Expression of human MICU1 with Flag tag in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    MICU1 (isoform 2) (MICU1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIND(V5)EcoDam

    Plasmid

    #59201

    Purpose

    Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Type

    Empty backbone

    Use

    Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGTM_COV2_NSP5_004_SUMO

    Plasmid

    #190062

    Purpose

    Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    3CLpro (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6X-His-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBAD_sfGFP3TCG4TAG

    Plasmid

    #174518

    Purpose

    Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    sfGFP
    

    Use

    Tags

    6 x His

    Expression

    Bacterial

    Mutation

    Recoded. TCG and TAG codons were introduced in po…

    Promoter

    araBAD

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRMC2-tat:msfGFP

    Plasmid

    #194915

    Purpose

    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR223-BRLF1(Rta)

    Plasmid

    #195824

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BRLF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BRLF1 (BRLF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-LMP2A-IRES-mCherry

    Plasmid

    #220353

    Purpose

    Expresses EBV latent gene LMP2A in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PLKO-mcherry-luc-puro-miR-211

    Plasmid

    #29784

    Depositor

    Carl Novina

    Article

    Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

    Insert

    miR-211
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMX-DivisionRecorder

    Plasmid

    #179446

    Purpose

    Retroviral expression vector containing the DivisionRecorder cassette

    Depositor

    Ton Schumacher

    Article

    Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

    Insert

    GFP-P2A-511-CrePI-511
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCSF107mT-GATEWAY-3'-Myc tag

    Plasmid

    #67617

    Purpose

    A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-terminal Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRMC2-sec:msfGFP

    Plasmid

    #194914

    Purpose

    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Str-STIM1-NN_ST-SBP-EGFP

    Plasmid

    #65262

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSG5-His-SUMO1

    Plasmid

    #17271

    Depositor

    Stephen Goff

    Article

    Yueh et al J Virol. 2006 Jan . 80(1):342-52.

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pXPR_123

    Plasmid

    #96919

    Purpose

    for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    p300

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RHBDD1 pmRFP-N2 (1390)

    Plasmid

    #62049

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    RHBDD1 (RHBDD1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pENTR-MECP2

    Plasmid

    #47806

    Purpose

    Expresses methyl-CpG binding protein 2 (Rett Syndrome)

    Depositor

    Huda Zoghbi

    Article

    Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    wild type

    Promoter

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pFUW-teto-mch-cFos

    Plasmid

    #193063

    Purpose

    doxycycline-inducible expression of mCherry fused to mouse cFos in mammalian cell

    Depositor

    Filipe Pereira

    Article

    Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

    Insert

    mch-cFos
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. xlox dNGFR-TERT

    Plasmid

    #69805

    Purpose

    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

    Depositor

    David Ott

    Article

    Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

    Insert

    hTERT reverse transcriptase (TERT Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mutation

    Promoter

    MLV promoter

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLgw EcoDam-V5-RFC1

    Plasmid

    #59209

    Purpose

    Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Type

    Empty backbone

    Use

    Lentiviral; Damid

    Tags

    Dam and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV hSyn hPGK1-mRuby

    Plasmid

    #220911

    Purpose

    Neuronal specific expression of the fusion construct PGK1-mRuby

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-mRuby (PGK1 Human)
    

    Use

    AAV

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLPC-FLAG.Mm.KAT5

    Plasmid

    #184647

    Purpose

    Retroviral expression of mouse Kat5

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Kat5 (Kat5 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pD4sE_SC12

    Plasmid

    #219721

    Purpose

    DV4 stable E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV4 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pD1sE_SC12

    Plasmid

    #219718

    Purpose

    DV1 E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV1 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…

    Promoter

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHLP700

    Plasmid

    #203742

    Purpose

    C-terminal tagging of target proteins with AID*/3xHA degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain codons 71-114
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MSCV-LMP1-IRES-mCherry

    Plasmid

    #220351

    Purpose

    Expresses EBV latent gene LMP1 in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR223-BMRF1(EA-D)

    Plasmid

    #195807

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BMRF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BMRF1 (BMRF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pB-Ric8b-Integration

    Plasmid

    #129457

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    Ric8b (Ric8b Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge