We narrowed to 152,943 results for: Des

1. pORANGE Tubb3-GFP KI

   Plasmid

   #131497

   Purpose

   Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pORANGE CACNG8-GFP KI #2

   Plasmid

   #131474

   Purpose

   Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cacng8 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223_Luciferase

   Plasmid

   #25894

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   Luciferase
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBbA5a-sufCDSUB

   Plasmid

   #195055

   Purpose

   Expresses SUF operon from B. subtilis from IPTG-inducible promoter

   Depositor

   Greg Bokinsky

   Article

   D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

   Insert

   sufCDSUB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTRY-GFP

   Plasmid

   #15301

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   hrGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

   Plasmid

   #131508

   Purpose

   Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBC

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV ORANGE Gria1-HaloTag KI

   Plasmid

   #139655

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-IP3KA

   Plasmid

   #133582

   Purpose

   Expresses IP3KA GFP tagged

   Depositor

   Christophe Erneux

   Article

   Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

   Insert

   Human inositol 1,4,5-trisphosphate 3-kinase A GFP tagged (ITPKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223_HcRed

   Plasmid

   #25892

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   hcRed
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pORANGE GFP-Clta KI

    Plasmid

    #131483

    Purpose

    Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Clta Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-IP3KB

    Plasmid

    #32528

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

    Insert

    IP3KB (ITPKB Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pORANGE GFP-Syt7 KI

    Plasmid

    #131488

    Purpose

    Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Syt7, Syt7 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTRY-YFP

    Plasmid

    #15302

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    YFP
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pORANGE GFP-Nlgn3 KI

    Plasmid

    #131501

    Purpose

    Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Nlgn3, Nlgn3 Rat, Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pORANGE Shank1-GFP KI

    Plasmid

    #131500

    Purpose

    Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Shank1, Shank1 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pORANGE Rims2-GFP KI

    Plasmid

    #131495

    Purpose

    Endogenous tagging of RIM2: C-terminal (amino acid position: S1551)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rims2, Rims2 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

    Plasmid

    #113677

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET19-MTdTc302

    Plasmid

    #207458

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc302 (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA

    Plasmid

    #113686

    Purpose

    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    KRAB

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-IP3KA

    Plasmid

    #32529

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

    Insert

    IP3KA (ITPKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only