We narrowed to 22,436 results for: Sele

1. pCas12b

   Plasmid

   #186449

   Purpose

   Encodes Cas12b from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12b
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas12b(PI)

   Plasmid

   #186451

   Purpose

   Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12b (PI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   R122A, G143P

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH

   Plasmid

   #148878

   Purpose

   Mammalian Expression of TNFalpha-ARE-polyA-Malat

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   TNFalpha-ARE-polyA-Malat (TNF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. iEscSnFR

   Plasmid

   #182807

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

   Insert

   iEscSnFR
   

   Use

   Tags

   6xHis, HA and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. iFluoxSnFR

   Plasmid

   #182808

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

   Insert

   iFluoxSnFR
   

   Use

   Tags

   6xHis, HA and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACE-K2P4.1-StrepTagII

   Plasmid

   #191474

   Purpose

   Expresses K2P4.1 with C-terminal StrepTagII

   Depositor

   Arthur Laganowsky

   Article

   Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

   Insert

   K2P4.1 (KCNK4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   N104Q N108Q

   Promoter

   polH

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLiP-G1G2

   Plasmid

   #188965

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. puc19_N_tag

   Plasmid

   #186650

   Purpose

   Empty donor plasmid.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   N-terminal 3xFlag-3xHA Tag
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-eGFP-OPTN S473A 513A

   Plasmid

   #175763

   Purpose

   Lentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513A

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S473A S513A

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIN-CD45

    Plasmid

    #175943

    Purpose

    knockout mouse CD45

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    CD45 gRNA (Ptprc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGG422_UV5

    Plasmid

    #165607

    Purpose

    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D1332K and R1333G mutations in SpCas9

    Promoter

    lacUV5 driving Cas9 KG and UV5 driving sgRNA

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-GFP-EBA175-Flag(P1424G)

    Plasmid

    #155128

    Purpose

    Expresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424G; insert codes for EBA175 residues 1284 to …

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-HA-PfROM4 (H578A)

    Plasmid

    #154858

    Purpose

    Expresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM4 (PF3D7_0506900 Synthetic)
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Histidine 578 changed to Alanine

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-HA-PfROM4 (G470A/G471N)

    Plasmid

    #154859

    Purpose

    Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM4 (PF3D7_0506900 Synthetic)
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Glycine 470 changed to Alanine and Glycine 471 ch…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-HA-PfROM1 (A144G/N145G)

    Plasmid

    #154873

    Purpose

    Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM1 (PF3D7_0506900 Synthetic)
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Alanine 144 changed to Glycine and Asparagine 145…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT171_GalL_A3AΔ178-BE3

    Plasmid

    #145121

    Purpose

    Expressing base editor A3AΔ178-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ178-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-178AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT170_GalL_A3AΔ182-BE3

    Plasmid

    #145120

    Purpose

    Expressing base editor A3AΔ182-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ182-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-182AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT169_GalL_A3AΔ186-BE3

    Plasmid

    #145119

    Purpose

    Expressing base editor A3AΔ186-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ186-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-186AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT166_GalL_A3A-BE3

    Plasmid

    #145116

    Purpose

    Expressing base editor A3A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT139_GalL_nCDA1-VRERBE3

    Plasmid

    #145102

    Purpose

    Expressing base editor nCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VRERBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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