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actc1b-mKate2-rab8b

Plasmid

#109658

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab8b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab8b (rab8b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (R544W)

Plasmid

#29531

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed arginine 544 to tryptophan

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 IFNG

Plasmid

#67056

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IFNG (IFNG Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGEM-mouse TCR zeta/2451

Plasmid

#11506

Depositor

Allan Weissman

Article

Weissman et al Science. 1988 Feb 26. 239(4843):1018-21.

Insert

TCR zeta (Cd247 Mouse)

Use

General cloning

Mutation

n/a

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
rat APOBEC1-EGFP

Plasmid

#112857

Purpose

plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Cdc42 T17N sp64T

Plasmid

#16672

Depositor

De-Li Shi

Article

Djiane et al Development. 2000 Jul . 127(14):3091-100

Insert

Cdc42 T17N (CDC42 Human)

Use

Xenopus expression

Mutation

T17N. Dominant negative.

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
pBGSA c-myc-FPR1

Plasmid

#62601

Purpose

Constitutive expression of c-myc-FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

Insert

FPR1 (FPR1 Human)

Tags

c-myc

Expression

Mammalian

Promoter

SRalpha

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-Tubulin-6

Plasmid

#55512

Purpose

Localization: Microtubules, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Tubulin (TUBA1B Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mPapaya-Tubulin-C-18

Plasmid

#56399

Purpose

Localization: Microtubules, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Tubulin (TUBA1B Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pBR322 hCDK4 promoter

Plasmid

#16571

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

CDK4 promoter (CDK4 Human)

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pHH0103_NR2E3_Hormone-recep

Plasmid

#109852

Purpose

Protein expression and purification of NR2E3_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR2E3_Hormone-recep (NR2E3 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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mPapaya1-Tubulin-6

Plasmid

#56653

Purpose

Localization: Microtubules, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Tubulin (TUBA1B Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pIS1 Cyclin D2 long-mut-miR15/16

Plasmid

#21647

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 long-mut-miR15/16 (CCND2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant miR15/16 microRNA binding sites

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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Luc-Nanog-3'UTR

Plasmid

#63893

Purpose

Luciferase reporter of mouse wildtype Nanog 3'UTR

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-hHIF1 alpha-IGd2

Plasmid

#22535

Purpose

To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

hypoxia inducible factor 1 alpha (HIF1A Human)

Expression

Mammalian

Available Since

Nov. 9, 2009

Availability

Academic Institutions and Nonprofits only
GST eIF2beta

Plasmid

#45900

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2S2 (Eif2s2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pX330_sgACTA2

Plasmid

#63712

Purpose

Express sgACTA2 in mammalian cells.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

ACTA2 (ACTA2 Mouse, Human)

Expression

Mammalian

Promoter

U6

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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PCDF1-ERBB4 (E872K)

Plasmid

#29543

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
CMV-CC2

Plasmid

#51221

Purpose

expression of amino acid 917-1040 of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

aa917-1040

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only