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Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

Plasmid

#218205

Purpose

Zebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-2.1prox1a

Use

Zebrafish expression

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCK033 p5E desma MCS

Plasmid

#195948

Purpose

Gateway p5E vector

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

desmin a regulatory elements (desma Zebrafish)

Use

Other

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-p2A-CreERT2-FRT-kan-FRT

Plasmid

#108454

Purpose

This plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

mCherry-p2a-CreERT2

Use

Cre/Lox

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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p5xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180477

Purpose

Inducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Inserts

5x QUAS-ZsGreen-P2A
mCherry

Use

Synthetic Biology

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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PUC57_BAG3wt-eGFP

Plasmid

#98182

Purpose

Codes for full length, GFP tagged, human wildtype Bag3

Depositor

Robert Bryson-Richardson

Article

Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

Insert

BAG3wt-eGFP (BAG3 Human)

Tags

GFP

Expression

Bacterial

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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p13xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180480

Purpose

Inducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

13x QUAS-ZsGreen-P2A

Use

Synthetic Biology

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-EGFP

Plasmid

#127591

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Use

Zebrafish expression

Expression

Mammalian

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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loxP-myctagBFP-STOP-loxP-mCherry-NTR

Plasmid

#108456

Purpose

This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

loxP-myctagBFP-STOP-loxP-mCherry-NTR

Use

Cre/Lox

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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rag2:mcherry_CE_pISceI

Plasmid

#223047

Purpose

Express m-Cherry gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

m-Cherry

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Plasmid

#180474

Purpose

Inducible gene expression vector pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

QFDBD-2xQFAD*-VP16*-EcR

Use

Synthetic Biology

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

Plasmid

#110201

Purpose

Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

LexPR transactivator - 2A - Cerulean

Tags

Cerulean

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pME map2k2a_s219D

Plasmid

#201058

Purpose

Gateway middle entry vector expressing constitutively active zebrafish map2k2a

Depositor

Sarah Childs

Article

Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

Insert

MAP2k2a (map2k2a Zebrafish)

Use

Zebrafish expression

Mutation

Mutation S219D, constitutively active

Promoter

none

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pQFDBD-2x AD*-VP16*, α-cry:EGFP

Plasmid

#180473

Purpose

Gene expression vector pQFDBD-2x AD*-VP16*, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

QFDBD-2xQFAD*-VP16*

Use

Synthetic Biology

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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p9xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180479

Purpose

Inducible gene expression vector p9xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

9x QUAS-ZsGreen-P2A

Use

Synthetic Biology

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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p17xQUAS-Luciferase, α-cry:mCherry

Plasmid

#180482

Purpose

Inducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Inserts

Luciferase
17x QUAS

Use

Synthetic Biology

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-Cavin4b-TurboID

Plasmid

#163851

Purpose

In vitro transcription of Cavin4b-TurboID. Parton lab clone JTA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4b (cavin4b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mKate2

Plasmid

#162624

Purpose

Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mKate2

Expression

Mammalian

Promoter

SP6

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCK069 desmaMCS:minprom_mApple,exorh:EGFP

Plasmid

#195982

Purpose

Expression construct

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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