We narrowed to 17,072 results for: Por

1. Proinsulin-NanoLuc in pLX304

   Plasmid

   #62057

   Purpose

   Luminescent reporter of insulin secretion

   Depositor

   David Altshuler

   Article

   Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

   Insert

   Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Inserted NanoLuc into C-peptide of mouse Ins2 cod…

   Promoter

   CMV

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Beatrix

   Plasmid

   #167528

   Purpose

   Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

   Depositor

   Gian Michele Ratto

   Article

   Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

   Insert

   Beatrix
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS1408

   Plasmid

   #29205

   Purpose

   Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple201 (SLC6A5 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-shMcu-1

   Plasmid

   #181868

   Purpose

   Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

   Depositor

   Hilmar Bading

   Article

   Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

   Insert

   mitochondrial calcium uniporter (Mcu Mouse)
   

   Use

   AAV, Adenoviral, and Mouse Targeting

   Tags

   hrGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-shMcu-2

   Plasmid

   #181867

   Purpose

   Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

   Depositor

   Hilmar Bading

   Article

   Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

   Insert

   mitochondrial calcium uniporter (Mcu Mouse)
   

   Use

   AAV, Adenoviral, and Mouse Targeting

   Tags

   hrGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174645

   Purpose

   Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
   

   Use

   Tags

   SSPB(micro)-Venus and iLID-mCherry

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. BII-BCA-Prom-ASCL2

   Plasmid

   #133394

   Purpose

   Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

   Depositor

   Blair Madison

   Article

   Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

   Insert

   destabilized tdTomato-NLS and destabilized NLS-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free) drives expressi…

   Available Since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-noSV40-humanCOL2A1enhancer

   Plasmid

   #173961

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Enhancer region from COL2A1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-noSV40-humanEC1.45

   Plasmid

   #173946

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. KCC2-pHext

    Plasmid

    #104076

    Purpose

    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

    Depositor

    Igor Medyna

    Article

    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Use

    Tags

    pHluorine tag inserted in the second putative tra…

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    UbC

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1097

    Plasmid

    #29028

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple201 (SLC6A5 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-Cre-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMS1430

    Plasmid

    #29225

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple170 (POGZ Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL(EF1a-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

    Plasmid

    #236279

    Purpose

    Lentiviral vector that can express hAqp1 with fkbp12 degron in mammalian cells. EF1a promoter. EGFP marker.

    Depositor

    Arnab Mukherjee

    Article

    Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.

    Insert

    human aquaporin 1 (AQP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag and fkbp12

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

    Plasmid

    #236278

    Purpose

    Lentiviral vector that can express doxycycline-inducible hAqp1 with fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP marker.

    Depositor

    Arnab Mukherjee

    Article

    Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.

    Insert

    human aquaporin 1 (AQP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag and fkbp12

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtight

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid

    #118269

    Purpose

    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

    Promoter

    T7 Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500

    Plasmid

    #92319

    Purpose

    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500

    Plasmid

    #92318

    Purpose

    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    MLV LTR

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Archon1-KGC-EGFP-ER2-WPRE

    Plasmid

    #108423

    Purpose

    Archon1 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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