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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,640 results for: dykddddk

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Showing: 2681 - 2700 of 8640 results

  1. pSLQ10530

    Plasmid
    #239266
    Purpose
    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell lines
    Depositor
    Stanley Qi
    Publication
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NES-dPspCas13b-EGFP-ABI
    Use
    CRISPR and Lentiviral
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    EF1a
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239266-plasmid-map-sequence-id-474747

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  3. CCR2-DuET

    Plasmid
    #213198
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR2 (CCR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    213198-plasmid-map-sequence-id-431752

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  4. OPRM1-DuET

    Plasmid
    #213361
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRM1 (OPRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213361-plasmid-map-sequence-id-431826

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  5. APJ-DuET

    Plasmid
    #213185
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    APJ (APLNR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213185-plasmid-map-sequence-id-430751

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  10. pCDZ2FE_Pogz_1-1410

    Plasmid
    #105674
    Purpose
    Expresses Flag-tagged full-length human POGZ protein
    Depositor
    Thanos Halazonetis
    Insert
    POGZ (POGZ Human)
    Tags
    2X Flag tag
    Expression
    Mammalian
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105674-plasmid-map-sequence-id-203198

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  11. Flag-mCherry-Binder

    Plasmid
    #179139
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
    Depositor
    Klaus Hahn
    Publication
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179139-plasmid-map-sequence-id-353408

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  13. pTM-BPL

    Plasmid
    #173766
    Purpose
    Fluorescent labeling of the nuclear envelope
    Depositor
    Shinji Sueda
    Publication
    Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PDGF receptor TM domain, Biotin protein ligase
    Tags
    FLAG and Signal peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    173766-plasmid-map-sequence-id-342142

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  16. pmiHiFiCas9

    Plasmid
    #164832
    Purpose
    miHiFiCas9 expressing plasmid
    Depositor
    Eugene Chen
    Publication
    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miHiFiCas9 (contains Brex27 at sequence 5031-5138).
    Use
    CRISPR
    Tags
    FLAG and NLS
    Expression
    Mammalian
    Mutation
    change R 691 to A
    Promoter
    CMV
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164832-plasmid-map-sequence-id-329428

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  17. pEXPqcxip-hFTSJ3-FLAG

    Plasmid
    #165463
    Purpose
    Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.
    Depositor
    Martin Dorf
    Publication
    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11
    Insert
    FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
    Use
    Retroviral; Gateway
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV/MSV, 5'LTR
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    165463-plasmid-map-sequence-id-327804

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  20. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid
    #164576
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Publication
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164576-plasmid-map-sequence-id-323657

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Showing: 2681 - 2700 of 8640 results