We narrowed to 7,773 results for: Pet2;

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pET22b-ecDHFR-dCys

Plasmid

#110541

Purpose

Bacterial protein of E.coli DHFR C85A/C152S mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

C85 mutated to A85, C152 mutated to S152

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP

Plasmid

#110828

Purpose

Bacterial expression of E.coli DHFR N23PP mutant

Depositor

Stephen Benkovic

Article

Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

Insert

ecDHFR

Expression

Bacterial

Mutation

N23 mutated to P23; a proline residue inserted be…

Promoter

T7

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b+R3(R112C/T180C)C

Plasmid

#108194

Purpose

Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C)-P450cam fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b+3(R112C/T180C)C

Plasmid

#108193

Purpose

Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA3(R112C/T180C)-P450cam fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-Metavinculin tail

Plasmid

#107152

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

Insert

metavinculin (VCL Human)

Tags

(His)8

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN01(∆M)-His

Plasmid

#84510

Purpose

E.coli version of EPN01(∆M)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN01(∆M)-His

Tags

His tag

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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csd2_121-308/pET21a(+)

Plasmid

#83293

Purpose

csd2, construct: 121-308, pET21a(+) no-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Expression

Bacterial

Mutation

deleted amino acid 1-120

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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csd5_41-192/pET28b(+)

Plasmid

#83296

Purpose

csd5, construct: 41-192, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1250

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-40

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd4_22-438/pET28b(+)

Plasmid

#83295

Purpose

csd4, construct: 22-438, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1075

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-21

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only
csd2_140-251/pET21a(+)

Plasmid

#83294

Purpose

csd2, construct:140-251, pET21a(+) C-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acids 1-139 and 252-308

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd1_54-312/pET21a(+)

Plasmid

#83291

Purpose

csd1, construct: 54-312, pET21a(+) C-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1543

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-53

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308/pET28b(+)

Plasmid

#83292

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd1_125-312/pET28b(+)

Plasmid

#83290

Purpose

csd1, construct: 125-312, pET28b(+) NC-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1543

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-124

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB(SnaBI)

Plasmid

#69558

Purpose

In vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

The two last codons (TATTAA; encoding the last ty…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBB(Y305P)

Plasmid

#69557

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

Codon TAT encoding tyrosine 305 was changed to CC…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET28-p53 (Cow 1-82)

Plasmid

#62062

Purpose

Expression of Cow p53 (residues 1-82) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-82

Promoter

T7

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-73) (K24N)

Plasmid

#62079

Purpose

Expression of human p53 (1-73) (K24N) in e. coli

Depositor

Gary Daughdrill

Article

Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-73; K24N

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET28-p53 (Rabbit 1-87)

Plasmid

#62058

Purpose

Expression of Rabbit p53 (residues 1-87) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-87

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (PPP)

Plasmid

#62067

Purpose

Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A, P27A

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET28-ceRab2(1-214)

Plasmid

#59550

Purpose

makes 6x Fusion of Full length ceRab2

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-214

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only