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Lenti-sgRasa1#2/Cre

Plasmid

#173650

Purpose

Expresses a Rasa1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Rasa1 (Rasa1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRasa1#1/Cre

Plasmid

#173649

Purpose

Expresses a Rasa1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Rasa1 (Rasa1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgStag2#1/Cre

Plasmid

#173659

Purpose

Expresses a Stag2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Stag2 (Stag2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgStag2#2/Cre

Plasmid

#173660

Purpose

Expresses a Stag2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Stag2 (Stag2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPole#1/Cre

Plasmid

#173609

Purpose

Expresses a Pole-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pole (Pole Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-NQO1(Y128F)

Plasmid

#177141

Purpose

Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

NQO1 (NQO1 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

Plasmid

#154778

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Tags

LaG17

Expression

Mammalian

Mutation

204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X)

Plasmid

#156412

Purpose

Gateway middle entry clone containing an 8X multimerized ETS#2 DNA binding site from the murine Dll4 intronic enhancer

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

murine Dll4 F2- ETS site B/ site #2, 8X

Use

Gateway cloning

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 5kb promoter/AcGFP

Plasmid

#98101

Purpose

Zebrafish aBb crystallin 5kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 5 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 5 kb promoter fragment …

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 4kb promoter/AcGFP

Plasmid

#98100

Purpose

Zebrafish aBb crystallin 4kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 4 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 4 kb promoter fragment …

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Nkx2-5YRD Y-A

Plasmid

#98611

Purpose

Gateway entry vector for NKX2-5 YRD Y-A

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 YRD Y-A (Nkx2-5 Mouse)

Use

Entry vector

Mutation

Substitutions of 7 tyr into Ala residues.Y233A; Y…

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00295

Plasmid

#34421

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12052 (lola Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00321

Plasmid

#34446

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8676 (Hr39 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00050

Plasmid

#34212

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11502 (svp Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00060

Plasmid

#34221

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9415 (Xbp1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00012

Plasmid

#34174

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8328 (HLHmdelta Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCS2+HyPer7-NLS

Plasmid

#136468

Purpose

Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Triple nuclear localization signal of SV40 (simia…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+MLS-HyPer7

Plasmid

#136470

Purpose

Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Tandem mitochondrial targeting signal of cytochro…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABE8e_dNRCH-BlastR

Plasmid

#232720

Purpose

A-to-G base editor

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

ABE8e-dCas9(NRCH)

Expression

Mammalian

Mutation

within Cas9 D10A H840A

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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LCV2_EGFP

Plasmid

#155098

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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