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ART

Plasmid

#214063

Purpose

Fluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-sgRNA

Plasmid

#177783

Purpose

Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-GFP-VHL

Plasmid

#21053

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pMB30

Plasmid

#122247

Purpose

Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-IPP1-His

Plasmid

#118978

Purpose

Bacterial expression plasmid for Inorganic pyrophosphatase

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

inorganic diphosphatase IPP1 (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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HH-gRNA-HDV-Shuttle-Vector

Plasmid

#169098

Purpose

Backbone plasmid to generate HH-gRNA-HDV segment

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

HH-gRNAbackbone-HDV

Expression

Mammalian

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP670-2-T2A-HO1

Plasmid

#197250

Purpose

Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP670-2-T2A-HO1

Expression

Worm

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-RanQ69L

Plasmid

#30309

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

RanQ69L (RAN N/A)

Tags

FLAG-Cherry

Expression

Mammalian

Mutation

Glutamine 69 is changed to Leucine

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-5-enzymatic-domain

Plasmid

#214306

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

aa133-434

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-SEP-GluA2 TKIT

Plasmid

#169442

Purpose

Expression of 2 guides + donor DNA

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

AAV and CRISPR

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluN2A TKIT donor

Plasmid

#169429

Purpose

Donor DNA for TKIT knockin of SEP to NR2A in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluN2B TKIT donor

Plasmid

#169431

Purpose

Donor DNA for TKIT knockin of SEP to NR2B in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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LRT_RTctrl

Plasmid

#214074

Purpose

Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LRT

Plasmid

#214071

Purpose

Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHFS:SEP-rGluA2 TKIT donor

Plasmid

#169440

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in rat

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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anti-ALFA_Nb_GFP

Plasmid

#244063

Purpose

fusion of the ALFA binder (nanobody) to sfGFP

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

anti-ALFA_Nb_sfGFP_GB1

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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Vimentin_mCherry_ALFA

Plasmid

#244059

Purpose

anchoring mCherry-1x ALFA to the intermediate filaments

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

NtVimentin-mCherry-ALFA

Tags

ALFA

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA2 TKIT donor

Plasmid

#169421

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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ART_RTctrl

Plasmid

#214066

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge