We narrowed to 9,607 results for: FIE

1. NET5L pEGFP-N2 (1155)

   Plasmid

   #62035

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   NET5L (TMEM201 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   long form

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. NKP68 RFP (790)

   Plasmid

   #62003

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

   Insert

   NKP68 (TMUB1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   pgk

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

3. EGFP-uTEV1-ACBD5

   Plasmid

   #171008

   Purpose

   HiLITR protease with ACBD5 C-terminal targeting information (peroxisome)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-ACBD5(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. POPDC2 pEGFP-N2 (1087)

   Plasmid

   #62030

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   POPDC2 (POPDC2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMP71-mtGFP-llo-lama4-alg8

   Plasmid

   #174605

   Purpose

   expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   palmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETNKI-strepII-3C-LIC-kan

   Plasmid

   #108706

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ATP1A1_G8_Dual_sgRNA

   Plasmid

   #178104

   Purpose

   Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-SP110A

   Plasmid

   #65392

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   SP110A
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

9. SRGN-bio-His

   Plasmid

   #52088

   Purpose

   Expresses full-length Serglycin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SRGN (SRGN Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p258 pSP72 hAR-LBD

    Plasmid

    #12492

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Insert

    AR LBD (AR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AR ligand binding domain

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pFastBac HT JS-Munc18b

    Plasmid

    #135554

    Purpose

    Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18b (Stxbp2 Rat)
    

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SEMA4-bio-His

    Plasmid

    #51827

    Purpose

    Expresses full-length Semaphorin-4D precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SEMA4 (SEMA4D Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EGFP-uTEV1-SQStmd

    Plasmid

    #171009

    Purpose

    HiLITR protease with SQS C-terminal targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-SQS(tmd)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe_puro_DEST_Flag_PRKCI

    Plasmid

    #45263

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PKCI (PRKCI Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

15. LentiCRISPRv2puro-Ptger1

    Plasmid

    #170306

    Purpose

    A knock-out vector for the mouse Ptger1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Ptger1 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. aav-U6-broccoli-hsp68-mCherry-MCS-pA

    Plasmid

    #195411

    Purpose

    AAV9 based enhancer activity assay

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3E-EHD2b

    Plasmid

    #109569

    Purpose

    Multisite gateway vector for 3' tagging with EHD2b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    EHD2b (ehd2 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-EF1-mouse SmyD1-T2A-copGFP

    Plasmid

    #101157

    Purpose

    Lentiviral expression of SmyD1 and copGFP

    Depositor

    Martine Roussel

    Article

    Wei et al Oncotarget. 2017 Jul 27. doi: 10.18632/o

    Insert

    SmyD1 (Smyd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. STIMATE(1-240)

    Plasmid

    #71280

    Purpose

    Expresses STIMATE or TMEM110 (1-240) in mammalian cells

    Depositor

    Yubin Zhou

    Article

    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

    Insert

    STIMATE (1-240) (TMEM110 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pBABE human IRS-1 CAAX

    Plasmid

    #11237

    Depositor

    Ronald Kahn

    Article

    Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    C-terminal prenylation motif makes IRS-1 localize…

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only