We narrowed to 6,648 results for: hwa

1. SR140

   Plasmid

   #156037

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SR140 (U2SURP Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV5-rat ERK2-WT

   Plasmid

   #40812

   Depositor

   Natalie Ahn

   Article

   Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

   Insert

   MAPK1 (Mapk1 Rat)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCarCBADE_ProB(I69E)A

   Plasmid

   #117756

   Purpose

   ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

   Depositor

   Greg Bokinsky

   Article

   Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

   Insert

   Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac(UV5)

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQlinkH-PTPIP51(36-235)

   Plasmid

   #170533

   Purpose

   Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH2R-mCherry-N1

   Plasmid

   #84326

   Purpose

   Histamine-2 receptor tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   H2R (HRH2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe(puro) mouse Egr3

   Plasmid

   #108102

   Purpose

   Retroviral vector for expression of mouse Egr3

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   Egr3 (Egr3 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLC242-GFP-metap2

   Plasmid

   #100518

   Purpose

   Expresses GFP tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   Metap2 (METAP2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-FLAG-Sestrin1 isoform1

   Plasmid

   #72594

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN1 (SESN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry

   Plasmid

   #83491

   Purpose

   cre-dependent DN-AMPK under EF1a promoter

   Depositor

   Dong Kong

   Article

   Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

   Insert

   DN-AMPK-2A-mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   K45R

   Promoter

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX317-hcRED

    Plasmid

    #115440

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    hcRED
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCVL.Active/Repressed TLR (Sce)

    Plasmid

    #45575

    Purpose

    Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE80L-SH3

    Plasmid

    #160331

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

    Insert

    SH3
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. THOC1

    Plasmid

    #156081

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    THOC1 (THOC1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1_VNp15-StefinA

    Plasmid

    #182405

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-stefin (CSTA Human)
    

    Use

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CABE-T2.19

    Plasmid

    #193265

    Purpose

    Expression of CABE-T2.19 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T2.19
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRevTRE MKL1-N100

    Plasmid

    #19848

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1-N100 (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    deleted amino acids 1-100

    Promoter

    Available Since

    Jan. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3 Flag MKK4(ala)

    Plasmid

    #15314

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

    Insert

    MKK4(ala) (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-220Ala Thr-224Ala

    Promoter

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

18. 3341 pcDNA3 Bcl-2 T69A S70A S87A

    Plasmid

    #13340

    Depositor

    Stanley Korsmeyer

    Article

    Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Threonine 69 to Alanine; changed Serine 7…

    Promoter

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

19. TTR C10S S85C

    Plasmid

    #184410

    Purpose

    bacterial expression of TTR

    Depositor

    Peter Wright

    Article

    Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S85C

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPAICS-2XStrep

    Plasmid

    #125683

    Purpose

    expresses human PAICS protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    PAICS (PAICS Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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