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pGB-As-NF-kB2

Plasmid

#180210

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Tags

GAL4 (aa 1-147) fusion protein

Expression

Yeast

Mutation

aa 2-502

Promoter

AdhI

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1351-1588_AE

Plasmid

#148630

Purpose

Bacterial Expression of HsNot1iso1_1351-1588

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot1iso1_1351-1588 (CNOT1 Human)

Expression

Bacterial

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1_1-177-YYMLtoAAAA_AE

Plasmid

#148575

Purpose

Mammalian Expression of HsGIGYF1_1-177-YYMLtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_1-177-YYMLtoAAAA (GIGYF1 Human)

Expression

Mammalian

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_52-234opt_AE

Plasmid

#148564

Purpose

Bacterial Expression of Hs4EHP_52-234opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt (EIF4E2 Human)

Expression

Bacterial

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-Co-Cterm

Plasmid

#180208

Purpose

Expresses GAL4 fusion of Capsaspora NF-kB C-terminal sequences in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-RHD

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 542-1224

Promoter

AdhI

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB3

Plasmid

#180211

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB3

Tags

GAL4 (aa 1-147) fusion protein

Expression

Yeast

Mutation

aa 2-412

Promoter

AdhI

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-As-NF-kB3

Plasmid

#180202

Purpose

Expresses FLAG-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB3

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-412

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Co-NF-kB-Cterm

Plasmid

#180199

Purpose

Expresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kBCterm

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 542-1224

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-M141R_R

Plasmid

#147379

Purpose

Mammalian Expression of HsNot7-M141R

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-M141R (CNOT7 Human)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot7_9-260_R

Plasmid

#147369

Purpose

Bacterial Expression of HsNot7_9-260

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7_9-260 (CNOT7 Human)

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCB’ CsoS2 ΔNTD

Plasmid

#162705

Purpose

Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Mutation

Deletion of residues 2-256 of CsoS2, Y131D

Promoter

PLtet0-1 promoter

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1-K77R

Plasmid

#174813

Purpose

Bacterial expression of inactive ALC1 (K77R disrupts ATP binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1-K77R (CHD1L Human)

Tags

GST

Expression

Bacterial

Mutation

K77R

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1-E175Q

Plasmid

#174814

Purpose

Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1-E175Q (CHD1L Human)

Tags

GST

Expression

Bacterial

Mutation

E175Q

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCCM’ CbbQ-

Plasmid

#162711

Purpose

Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Expression

Bacterial

Mutation

Inactivation of CbbQ subunit of CbbOQ rubisco act…

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCCM’ ΔacRAF

Plasmid

#162710

Purpose

Second CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAF

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Expression

Bacterial

Mutation

pCCM' with a deletion of putative rubisco ch…

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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myc-Miro2 (lacking TM)

Plasmid

#127614

Purpose

Human Miro2 with the transmembrane deleted

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Miro2 (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

Transmembrane deletion

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUL33

Plasmid

#135103

Purpose

Codon-optimized HSV-1 UL33 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Tags

HA-Strep-Strep

Expression

Mammalian

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUL50-CStHA

Plasmid

#135105

Purpose

Codon-optimized HSV-1 UL50 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL50 codon optimized

Tags

Strep-Strep-HA

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUL33-CStHA

Plasmid

#135106

Purpose

Codon-optimized HSV-1 UL33 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Tags

Strep-Strep-HA

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUS2-CStHA

Plasmid

#135107

Purpose

Codon-optimized HSV-1 US2 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Tags

Strep-Strep-HA

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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