We narrowed to 17,403 results for: EMB

1. pQHA-USP11 WT puroR

   Plasmid

   #46749

   Purpose

   Retroviral vector that expresses wild type HA-tagged wild type human USP11

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-MS2-HA-HsRck_AC

   Plasmid

   #148417

   Purpose

   Mammalian Expression of HsRck

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsRck (DDX6 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQHA-USP11 CS puroR

   Plasmid

   #46750

   Purpose

   Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C318S--catalytically inactive

   Promoter

   CMV

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. RepairTemplate_NIPBL_mEGFP_mAID

   Plasmid

   #217664

   Purpose

   Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   NIPBL_mEGFP_mAID (NIPBL Human)
   

   Tags

   mAID and meGFP

   Expression

   Bacterial and Mammalian

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-EGFP-SOS1

   Plasmid

   #178860

   Purpose

   Mammalian expression of SOS1 fused to EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   SOS1 (SOS1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-IRAlus-Lin28

   Plasmid

   #92347

   Purpose

   Plasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.

   Depositor

   Ling-Ling Chen, Gordon Carmichael

   Article

   Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

   Insert

   Lin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330a dCas9-VP64

   Plasmid

   #92363

   Purpose

   Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pclbw-eGFPomp25

   Plasmid

   #58424

   Purpose

   Mammalian expression of GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   eGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   CMV/b-actin

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pBTK552

   Plasmid

   #110618

   Purpose

   BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI.

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pEGFP-Alu-Lin28

    Plasmid

    #92346

    Purpose

    Plasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.

    Depositor

    Ling-Ling Chen, Gordon Carmichael

    Article

    Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

    Insert

    Lin28 gene derived Alu element containing 3'UTR (LIN28A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-PBD

    Plasmid

    #60880

    Purpose

    Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA365-653

    Depositor

    Trina Schroer

    Article

    Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

    Insert

    Polo-like kinase 1 (PLK1 BC01486, Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pRDB_181

    Plasmid

    #216102

    Purpose

    CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPRv1 sgTollip

    Plasmid

    #196546

    Purpose

    Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDA_662

    Plasmid

    #216101

    Purpose

    CRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRDA_831

    Plasmid

    #216100

    Purpose

    CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEMHE:mTREK-1(tandem wt-wt)

    Plasmid

    #224757

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(tandem wt-wt)

    Depositor

    Dan Minor

    Article

    Bagriantsev et al EMBO J. 2012 Aug 1;31(15):3297-308. doi:

    Insert

    Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Promoter

    T7

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV10.KH

    Plasmid

    #63208

    Purpose

    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57-CMV-MAAP-SL08

    Plasmid

    #218247

    Purpose

    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein Variant SL08 driven by CMV promoter
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5HT6-YFP-ThymosinBeta4(WT)

    Plasmid

    #96806

    Purpose

    cilia-membrane-targeted G-actin-binding protein

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. RHBDD1 pmRFP-N2 (1390)

    Plasmid

    #62049

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    RHBDD1 (RHBDD1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only