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pGMCtN-P38-luxAB2

Plasmid

#27065

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB integrative

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pMPO 54

Plasmid

#69865

Purpose

Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-MCSII, M13 replication origin

Depositor

Eduardo Santero

Article

Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pUC-Leum

Plasmid

#44364

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

Leum promoter

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pTE-0X38-GCT6

Plasmid

#27057

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp-DAS+4

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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ZE9 tiggywinkle hedgehog pT7TS

Plasmid

#17058

Depositor

Randall Moon

Article

Ekker et al Curr Biol. 1995 Aug 1. 5(8):944-55.

Insert

tiggywinkle hedgehog (shhb Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pHREP-KD-2-shH1

Plasmid

#241994

Purpose

Sleeping Beauty vector for inducible expression of a chicken H1 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H1

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH3

Plasmid

#241995

Purpose

Sleeping Beauty vector for inducible expression of a chicken H3 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H3

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH4

Plasmid

#240431

Purpose

Sleeping Beauty vector for inducible expression of a chicken H4 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H4

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-LP

Plasmid

#240432

Purpose

Empty landing pad vector with two Bxb1 recombination sites for RMCE and LNGFR + EGFP-HygroR for sorting/selection.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Inserts

LNGFR
EGFP

Tags

Hygromycin Resistance

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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SM468_EF1A-dHyperLbCas12a-T2A-EGFP

Plasmid

#244220

Purpose

Mammalian expression of dHyperLbCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM471_EF1A-dEnAsCas12a-T2A-BlastR

Plasmid

#244223

Purpose

Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM469_EF1A-dHyperLbCas12a-T2A-BlastR

Plasmid

#244221

Purpose

Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCR158

Plasmid

#240639

Purpose

Expression of NLS-Kaede in Exaiptasia diaphana

Depositor

John Pringle

Inserts

AIPGENE865 promoter
Kaede

Use

Expression of nls-kaede in exaiptasia diaphana

Tags

SV40 NLS

Mutation

naturally occurring 749-bp insertion at bp 628 (f…

Promoter

Exaiptasia diaphana Elongation Factor 1 alpha (AI…

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKDC.100

Plasmid

#222224

Purpose

Acceptor plasmid for yeast retron-Eco1 ncRNA variant libraries.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Type

Empty backbone

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_ZF1_mNeonGreen

Plasmid

#213834

Purpose

Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_mNeonGreen_ZF2

Plasmid

#213835

Purpose

Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_ZF2_mNeonGreen

Plasmid

#213836

Purpose

Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen_ZF1

Plasmid

#213837

Purpose

Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_mNeonGreen

Plasmid

#213838

Purpose

Facilitates genomic integration of the control reporter without zinc finger binding sites.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-2-VP16

Plasmid

#213842

Purpose

Used for expressing VP16 fused to ZF-2.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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