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pMPO 54

Plasmid

#69865

Purpose

Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-MCSII, M13 replication origin

Depositor

Eduardo Santero

Article

Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGMCtN-P38-luxAB2

Plasmid

#27065

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB integrative

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pUC-Leum

Plasmid

#44364

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

Leum promoter

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pTE-0X38-GCT6

Plasmid

#27057

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp-DAS+4

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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ZE9 tiggywinkle hedgehog pT7TS

Plasmid

#17058

Depositor

Randall Moon

Article

Ekker et al Curr Biol. 1995 Aug 1. 5(8):944-55.

Insert

tiggywinkle hedgehog (shhb Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCR158

Plasmid

#240639

Purpose

Expression of NLS-Kaede in Exaiptasia diaphana

Depositor

John Pringle

Inserts

AIPGENE865 promoter
Kaede

Use

Expression of nls-kaede in exaiptasia diaphana

Tags

SV40 NLS

Mutation

naturally occurring 749-bp insertion at bp 628 (f…

Promoter

Exaiptasia diaphana Elongation Factor 1 alpha (AI…

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKDC.100

Plasmid

#222224

Purpose

Acceptor plasmid for yeast retron-Eco1 ncRNA variant libraries.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Type

Empty backbone

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_ZF1_mNeonGreen

Plasmid

#213834

Purpose

Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_mNeonGreen_ZF2

Plasmid

#213835

Purpose

Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_ZF2_mNeonGreen

Plasmid

#213836

Purpose

Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen_ZF1

Plasmid

#213837

Purpose

Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_mNeonGreen

Plasmid

#213838

Purpose

Facilitates genomic integration of the control reporter without zinc finger binding sites.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-2-VP16

Plasmid

#213842

Purpose

Used for expressing VP16 fused to ZF-2.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1-VP16

Plasmid

#213843

Purpose

Used for expressing VP16 fused to ZF-1.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-STReTCh-RGD

Plasmid

#216532

Purpose

Expresses HaloTag-STReTCh-RGD fusion protein in bacteria

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

HaloTag-STReTCh-RGD

Tags

6xHis and HaloTag

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pRegev_D3

Plasmid

#204182

Purpose

Single reporter with long guanine depleted (G-poor) region from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D1.61C>G

Plasmid

#204179

Purpose

pRegev_D1 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D1 with 61C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

61C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S5

Plasmid

#204174

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S5 in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D2.30C>G

Plasmid

#204181

Purpose

pRegev_D2 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D2 with 30C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

30C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S1

Plasmid

#204150

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S1 exon in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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