We narrowed to 208 results for: pet cas9
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Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyExpressionBacterialPromoterEc-TTL-P112 and Ec-TTL-P104Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE3∆UGI
Plasmid#96943PurposeThe plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).DepositorInsertBE3∆UGI
TagsHis6 tagExpressionBacterialPromoterT7Available SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Hf-RfxCas13d-His
Plasmid#234480PurposePlasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)DepositorInsertRfxCas13d
UseCRISPRTags6xHisExpressionBacterialMutationChanged four alanine to valine in positions 134, …Available SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L
Plasmid#89610PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416DepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)
Plasmid#114077PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R/S542R/K607RAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-4F2-mCherry
Plasmid#73421PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.DepositorInsertPromoter 4F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP4F2 (orthogonal T7-lac variant)Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)
Plasmid#114075PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R, S542R, K548V and N552RAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)
Plasmid#114071PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R and S542RAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
Tags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L-MM
Plasmid#89611PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416DepositorAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only