We narrowed to 3,017 results for: PPL

1. iD20 LoxStopLox-Zim3-dCas9-mApple-hygro

   Plasmid

   #204720

   Purpose

   Cre-inducible CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

   Plasmid

   #182854

   Purpose

   Hetero-tetrmer expression vector with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

   Insert

   myr-palm-mEYFP-mCherry2-mEGFP-mApple
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP(1-233)-mApple(2-236)-HRas(2-189)

   Plasmid

   #133855

   Purpose

   Mammalian expression of HRas N-terminally tagged with EGFP and mApple

   Depositor

   Dave Piston

   Article

   Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

   Insert

   HRas (HRAS Human)
   

   Use

   Tags

   EGFP and mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

   Plasmid

   #196250

   Purpose

   Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL1-GlpT-scGFP-attL2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET PPL His6 MBP LIC cloning vector (2K-T)

   Plasmid

   #37183

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6, MBP, and PPL

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

   Plasmid

   #217970

   Purpose

   Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

   Depositor

   Robert Piper

   Insert

   2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

   Plasmid

   #204724

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

   Plasmid

   #217971

   Purpose

   Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.

   Depositor

   Robert Piper

   Insert

   2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

   Plasmid

   #216694

   Purpose

   Synchronize trafficking of GPI-AP from the ER (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   SBP-mApple-FIREtag-GPI
   

   Use

   Tags

   FIREtag, IL2 signal sequence, Streptavidin Bindin…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid

    #216695

    Purpose

    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    

    Use

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid

    #105842

    Purpose

    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMBPcyto-TEV-BseRI

    Plasmid

    #157739

    Purpose

    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Use

    Tags

    His10 + MBP (cytoplasmic expression; no signal se…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH_35S/mEGFP

    Plasmid

    #135321

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB_35S/mEGFP

    Plasmid

    #135320

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pN_35S/mEGFP

    Plasmid

    #132565

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. GS2.1/EC

    Plasmid

    #132568

    Purpose

    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    egg cell promoter, Cas9-mApple, pds3 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    A. thaliana egg cell promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Anti-Jagged [D5] (Recombinant Antibody)

    Antibody

    #201864-rAb

    Purpose

    Anti-Jagged (Human) recombinant scFv-Fc-fusion

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Recommended Applications

    Immunocytochemistry

    Reactivity

    Human

    Source Species

    Human

    Isotype

    IgG1

    Trial Size

    Available to purchase

    Available since

    Nov. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

18. Anti-Desmin [D7] (Recombinant Antibody)

    Antibody

    #201865-rAb

    Purpose

    Anti-Desmin (Human) recombinant scFv-Fc-fusion

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Recommended Applications

    ELISA

    Reactivity

    Human

    Source Species

    Human

    Isotype

    IgG1

    Trial Size

    Available to purchase

    Available since

    Oct. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

19. pRSII42K

    Plasmid

    #194523

    Purpose

    High copy vector backbone with G418 selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSII41K

    Plasmid

    #194522

    Purpose

    Low copy vector backbone with G418 selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge