Addgene

We narrowed to 878 results for: RK2

Showing: 261 - 280 of 878 results

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FLAG-MARK2-K82H-CR

Plasmid

#229425

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK2-K82H (MARK2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Kinase-dead mutant K82H; silent mutation CRISPR r…

Promoter

EFS

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK2-K168E

Plasmid

#199642

Purpose

Expresses kinase-inactive VRK2 (K168E)

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK2 (VRK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

K168E

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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LRRK2 gRNA (BRDN0001144775)

Plasmid

#77331

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRRK2 (LRRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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VRK2 gRNA (BRDN0001146649)

Plasmid

#77232

Purpose

3rd generation lentiviral gRNA plasmid targeting human VRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

VRK2 (VRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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VRK2 gRNA (BRDN0001145671)

Plasmid

#77233

Purpose

3rd generation lentiviral gRNA plasmid targeting human VRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

VRK2 (VRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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VRK2 gRNA (BRDN0001145054)

Plasmid

#77234

Purpose

3rd generation lentiviral gRNA plasmid targeting human VRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

VRK2 (VRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NMRK2 gRNA (BRDN0001147024)

Plasmid

#77128

Purpose

3rd generation lentiviral gRNA plasmid targeting human NMRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NMRK2 (NMRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NMRK2 gRNA (BRDN0001146501)

Plasmid

#77129

Purpose

3rd generation lentiviral gRNA plasmid targeting human NMRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NMRK2 (NMRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NTRK2 gRNA (BRDN0001146295)

Plasmid

#76469

Purpose

3rd generation lentiviral gRNA plasmid targeting human NTRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NTRK2 (NTRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5-ratERK2-I84A

Plasmid

#40815

Depositor

Natalie Ahn

Article

Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

I84A

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pBACgus/LRRK2 C-His_T2031E

Plasmid

#40379

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Expression

Mutation

aa1867-2186, T2031E

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Article

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Use

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-GRK2RH-Nluc

Plasmid

#158605

Purpose

Plasmid expressing GRK2(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]

Plasmid

#229387

Purpose

XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropout

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-Erk2

Plasmid

#50848

Purpose

Fluorescent reporter for Erk nuclear translocation

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

TagBFP

Expression

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP11-LRRK2_G2019S

Plasmid

#231175

Purpose

Expresses GFP-11-LRRK2 G2019S in mammalian cells

Depositor

Samara Reck-Peterson

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

GFP11-LRRK2 (G2019S) (LRRK2 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

G2019S

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPuro3.1(+)_Strep-Lrrk2-Myc

Plasmid

#161583

Purpose

expression of full-length mouse Lrrk2 with C-terminal Myc tag

Depositor

Maik Hintze

Insert

Lrrk2 (Lrrk2 Mouse)

Use

Tags

Myc tag and StrepII

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-MEK1_fusion

Plasmid

#39194

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-3XKD/Y1699C

Plasmid

#25367

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)_Strep-Lrrk2-Myc

Plasmid

#161582

Purpose

expression of full-length mouse Lrrk2 with C-terminal Myc tag

Depositor

Maik Hintze

Insert

Lrrk2 (Lrrk2 Mouse)

Use

Tags

Myc tag and StrepII

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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