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ssFv_lenti_wtATMSPARK2
Plasmid#195249Purposeexpress wtATMSPARK2 in a lenti vectorDepositorInsertATMSPARK2 sensor
UseLentiviralTagsExpressionMammalianMutationPromoterssfvAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
EE-LRRK2
Plasmid#193583PurposeExpresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminusDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsFYVE-CC2 and 3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRK248
Plasmid#173755Purposeco-injection plasmidDepositorInsertPrps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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ARK2aa
Plasmid#81013PurposeE.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-aphA
and PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSRK2721
Plasmid#78572PurposeGateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusionsDepositorInsertGateway cloning cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
ssFv_lenti_mutATMSPARK2
Plasmid#195250Purposeexpress Thr to Ala mutant ATMSPARK2 in a lenti vectorDepositorInsertThr to Ala mutant ATMSPARK2
UseLentiviralTagsExpressionMammalianMutationPromoterssfvAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRK261
Plasmid#173754PurposeCeLINC plasmidDepositorInsert(empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJRK249
Plasmid#173747PurposeCeLINC plasmidDepositorInsertPrps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterPrps-0Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_LRRK2-3
Plasmid#199272PurposepegRNA used to induce LRRK2 (c. 6055 G > A) mutationDepositorInsertLRRK2 6055GtoA pegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRK260
Plasmid#173753PurposeCeLINC plasmidDepositorInsertPrps-0::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterPrps-0Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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LRRK2_Halo_N_allele
Plasmid#178142PurposeDonor vector for endogenous tagging of human LRRK2 at the N-terminus with halotagInsertHalotag (LRRK2 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
DYRK2_HUMAN_D0
Plasmid#79729PurposeThis plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDYRK2 (DYRK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
VRK2_HUMAN_D0
Plasmid#79737PurposeThis plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVRK2 (VRK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJRK246
Plasmid#173741Purposedonor plasmid for SapTrap assemblyDepositorInsertwrt-2 promoter
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK253
Plasmid#173750PurposeCeLINC plasmidDepositorInsertPhsp-16.48::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterPhsp-16.48Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only
