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pTH1-SaSrtA-KQ
Plasmid#64982PurposeExpresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108QPromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB38-HF380deltaMTBD
Plasmid#37333DepositorInsertCytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)
UseDictyostelium expressionTagsHis6 and FLAG tagExpressionMutationdeleted amino acids 1-1387 and replaced amino aci…PromoterTRE-PminAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
MT363
Plasmid#65858PurposeThe gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.DepositorInsertnahG
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.7)-BE3
Plasmid#108380PurposeMammalian expression vector for xCas9(3.7)-BE3DepositorInsertxCas9(3.7)-BE3
UseTagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.7)-BE4
Plasmid#108381PurposeMammalian expression vector for xCas9(3.7)-BE4DepositorInsertxCas9(3.7)-BE4
UseTagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.6)-BE4
Plasmid#108386PurposeMammalian expression vector for xCas9(3.6)-BE4DepositorInsertxCas9(3.6)-BE4
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.6)-BE3
Plasmid#108385PurposeMammalian expression vector for xCas9(3.6)-BE3DepositorInsertxCas9(3.6)-BE3
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
p045
Plasmid#70057PurposeCo-expresses Syt1-SNARE37aa-linker complex with pDC184DepositorInsertsC2AB-linker-SNAP-25_C
SNAP-25_N
UseTagsNo tagsExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDC184
Plasmid#70055PurposeCo-expresses Syt1-SNARE37aa-linker complex with p045DepositorInsertsHis tagged Synaptobrevin 28-89
Syntaxin 191-256
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Alpha-Actinin-19
Plasmid#57346PurposeLocalization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEos2-Alpha-Actinin-C-14
Plasmid#57347PurposeLocalization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
ReaLiSM A1-Hinge-GPIba+2cys
Plasmid#59581Purposeexpress VWF A1-GPIba ReaLiSM construct in mammalian cellsDepositorInsertVWF A1-linker-GPIba (VWF Human)
UseTags6HisExpressionMammalianMutationPromoterchick beta-actinAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
