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Plasmid#204434PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
UseTagsEGFPExpressionMammalianMutationF860A Y861A Y862A Y863A Q864A R865A H866A F867APromoterCMVAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_alpha-domain
Plasmid#200302PurposeBacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2aDepositorInsertalpha-domain of metallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 3
Plasmid#188363PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant3-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant3-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-SpGalpha-i
Plasmid#185568Purposeto see protein dynamicsDepositorInsertGalpha-i
UseIn vitro transcription (mrna synthesis)TagsGFP, S-TagExpressionMutationPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-nE-mHP1alpha
Plasmid#181895PurposeFluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamatesDepositorInsertnE-mHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionMammalianMutationS11E, S12E, S13E, S14EPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pColE1-AlphaOmega
Plasmid#165867PurposeTerminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Alpha1
Plasmid#165858PurposeAlpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha2
Plasmid#165863PurposeAlpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAT2_G-alpha
Plasmid#109947PurposeProtein expression and purification of GNAT2_G-alphaDepositorInsertGNAT2_G-alpha (GNAT2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GGA3_Alpha-adaptinC2
Plasmid#109926PurposeProtein expression and purification of GGA3_Alpha-adaptinC2DepositorInsertGGA3_Alpha-adaptinC2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AlpJ
Plasmid#139598Purposeexpresses AlpJ in E. coliDepositorInsertalpJ
UseTagsN terminal hexahistidineExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::galP_NS1
Plasmid#101843Purposedrives expression of galP transporter from NS1 locusDepositorInsertPtrc promoter driving GalP transporter expression
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha1_SF (GB0106)
Plasmid#68232PurposeTwister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScalps_puro_mIL-2Rα ST
Plasmid#59919PurposeExpresses mouse interleukin-2 receptor alpha chain mutatedDepositorInsertinterleukin 2 receptor alpha (Il2ra Mouse)
UseLentiviralTagsExpressionMutationchanged sequence of the intracellular (C-terminal…PromoterAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET16b-alpha3D
Plasmid#53310Purposebacterial expression of alpha3DDepositorInsertalpha3D
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRalpha shRNA #1
Plasmid#32619DepositorInsertTRalpha shRNA #1 (THRA Chicken)
UseRNAiTagsExpressionMutationPromoterAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
Comp AlphaPS3
Plasmid#14071DepositorInsertDrosphila Comp Alpha PS3 Integrin (scb Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS598 EF1alpha-EGFPcre
Plasmid#11923PurposeExpresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.DepositorInsertEGFP-cre (cre bacteriophage P1)
UseCre/LoxTagsEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
mGarnet2-alpha-tubulin
Plasmid#104318Purposecodes for a fusion of fluorescent protein mGarnet2 and alpha-tubulin, visualizes alpha-tubulinDepositorInsertmGarnet2-alpha-tubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Alpha-Actinin2-N-18
Plasmid#53988DepositorInsertAlpha-Actinin2
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits