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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,112 results for: cas12a

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Showing: 261 - 280 of 1112 results

  2. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid
    #155050
    Purpose
    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    Use
    CRISPR
    Available since
    Oct. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155050-plasmid-map-sequence-id-315172

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  3. Topo_SpCas9.tracr_LbCas12a.DR

    Plasmid
    #155049
    Purpose
    TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
    Use
    CRISPR
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155049-plasmid-map-sequence-id-308669

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  6. 6xHis-MBP-huMlCas12a

    Plasmid
    #174678
    Purpose
    Expresses 6xHis-MBP tagged MlCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huMlCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    174678-plasmid-map-sequence-id-346177

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  7. 6xHis-MBP-huCMaCas12a

    Plasmid
    #174679
    Purpose
    Expresses 6xHis-MBP tagged CMaCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huCMaCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174679-plasmid-map-sequence-id-346182

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  11. pCAG-AsCas12a-Plus-mCherry

    Plasmid
    #191231
    Purpose
    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity
    Depositor
    Zhili Rong
    Publication
    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AsCas12a-Plus-P2A-mCherry
    Use
    CRISPR
    Mutation
    E174R, R951K, R955A
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191231-plasmid-map-sequence-id-392988

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  13. pCMV-dCas12a-ABE8e

    Plasmid
    #193645
    Purpose
    Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-TadA*8e, EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    193645-plasmid-map-sequence-id-421948

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  16. PET28-FnCas12a-EP16

    Plasmid
    #183636
    Purpose
    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.
    Depositor
    Yunzi Luo
    Publication
    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-
    Insert
    FnCas12a-EP16
    Use
    CRISPR
    Tags
    6×His Tag
    Expression
    Bacterial
    Mutation
    N607R, K613V, N617R, K180S, K660R, D616N
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183636-plasmid-map-sequence-id-375575

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  19. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid
    #140220
    Purpose
    Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1
    Use
    CRISPR
    Tags
    miniVPR-mCherry
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140220-plasmid-map-sequence-id-291993

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Showing: 261 - 280 of 1112 results