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We narrowed to 5,224 results for: golden gate

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Showing: 261 - 280 of 5224 results

  2. The Mammalian Toolkit

    Plasmid Kit
    #1000000180
    Purpose
    Plasmids for Golden Gate-based assembly of cellular circuits for use in mammalian cell.
    Depositor
    Hana El-Samad
    Application
    Cloning and Synthetic Biology
    Vector Type
    Mammalian Expression
    Cloning Type
    Golden Gate
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only

  3. pICSL61003

    Plasmid
    #245680
    Purpose
    Level 0 Golden Gate part 3' Extensin-Full length-IEU (TerP1) terminator P1, GCTT - TAGA
    Depositor
    Mark Youles
    Insert
    3' Extensin-Full length-IEU (TerP1) terminator P1
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245680-plasmid-map-sequence-id-495766

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  4. pICSL41264_CP

    Plasmid
    #245692
    Purpose
    Level 0 Golden Gate Signal Peptide + Coding Sequence acceptor with AmilCP negative selection cassette AGGT - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245692-plasmid-map-sequence-id-495765

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  5. pICSL41308_CP

    Plasmid
    #245695
    Purpose
    Level 0 Golden Gate Coding Sequence (WITH stop codon) acceptor with AmilCP negative selection cassette AATG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245695-plasmid-map-sequence-id-495761

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  6. pICSL12049

    Plasmid
    #245646
    Purpose
    Level 0 Golden Gate part Double (enhancer)-34S FMV (Figwort Mosaic Virus). promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    Double (enhancer)-34S FMV (Figwort Mosaic Virus). promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245646-plasmid-map-sequence-id-495542

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  7. pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid
    #50627
    Purpose
    pRipTAL plasmid compatible with Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50627-plasmid-map-sequence-id-79594

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  8. pCB1532S-RFP

    Plasmid
    #101856
    Purpose
    Golden-Gate compatible fungal transformation vector carrying Sulfonylurea Resistance (Chlorimuron Ethyl) with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Article
    Pennington et al Figshare (2017)
    Type
    Empty backbone
    Use
    Fungal expression plasmid, tested in magnaporthe
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    101856-plasmid-map-sequence-id-197099

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  9. pICSL41308-LC

    Plasmid
    #245696
    Purpose
    Level 0 Golden Gate Coding Sequence (WITH stop codon) acceptor, Low - copy with pBR322 origin of replication AATG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245696-plasmid-map-sequence-id-495764

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  10. pICSL01005_CP

    Plasmid
    #245689
    Purpose
    Level 0 Golden Gate Coding Sequence (WITHOUT stop codon) acceptor with AmilCP negative selection cassette AATG - TTCG
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245689-plasmid-map-sequence-id-495758

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  11. pICSL60027

    Plasmid
    #245675
    Purpose
    Level 0 Golden Gate part Extensin-Truncated-EU + NbACT + Rb7MAR (D144-437 variant) terminator, GCTT - CGCT
    Depositor
    Mark Youles
    Insert
    Extensin-Truncated-EU + NbACT + Rb7MAR (D144-437 variant) terminator
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245675-plasmid-map-sequence-id-495497

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  13. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  14. pICSL01005-LC

    Plasmid
    #245697
    Purpose
    Level 0 Golden Gate Coding Sequence (WITHOUT stop codon) acceptor, Low - copy with pBR322 origin of replication AATG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245697-plasmid-map-sequence-id-495759

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  15. pCB1532H-RFP

    Plasmid
    #101855
    Purpose
    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Article
    Pennington et al Figshare (2017)
    Type
    Empty backbone
    Use
    Fungal expression plasmid, tested in magnaporthe
    Available Since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    101855-plasmid-map-sequence-id-195301

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  16. pICSL12047

    Plasmid
    #245644
    Purpose
    Level 0 Golden Gate part SGT-34S FMV (Figwort Mosaic Virus). Sub-Genomic Transcript (SGT) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    SGT-34S FMV (Figwort Mosaic Virus). Sub-Genomic Transcript (SGT) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245644-plasmid-map-sequence-id-495540

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  17. GoldenPiCS Kit

    Plasmid Kit
    #1000000133
    Purpose
    A flexible modular system for advanced strain engineering in Pichia pastoris to accomplish pathway expression, protein production, or other DNA integration applications.
    Depositor
    Brigitte Gasser
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pCB1532B-RFP

    Plasmid
    #101854
    Purpose
    Golden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Article
    Pennington et al Figshare (2017)
    Type
    Empty backbone
    Use
    Fungal expression plasmid, tested in magnaporthe
    Available Since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    101854-plasmid-map-sequence-id-204750

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  19. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198048
    Purpose
    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198048-plasmid-map-sequence-id-389382

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  20. pL1P5 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198046
    Purpose
    MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198046-plasmid-map-sequence-id-383687

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Showing: 261 - 280 of 5224 results