Addgene

We narrowed to 3,071 results for: golden gate

Showing: 261 - 280 of 3071 results

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p5E_GGWDest-

Plasmid

#49318

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pME_GGWDest+

Plasmid

#49321

Purpose

Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGDestSC-ATG

Plasmid

#49322

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_XcmI-LacZ

Plasmid

#49296

Purpose

Golden Gate entry vector at position 1, no 5' ATG, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGW_SM_BglII

Plasmid

#49314

Purpose

Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E_GGWDest+

Plasmid

#49317

Purpose

Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_GGWDest-

Plasmid

#49320

Purpose

Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_Linker

Plasmid

#49281

Purpose

Golden Gate entry vector at position 1, small linker insert, no 5' ATG

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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mUAV

Plasmid

#102680

Purpose

Mobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable parts

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:amilCP:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMA_Level 1A

Plasmid

#102701

Purpose

Mobius Assembly Level 1 Acceptor Vector A

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:spisPink:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Level 2A

Plasmid

#102705

Purpose

Mobius Assembly Level 2 Acceptor Vector A

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:sfGFP:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL001

Plasmid

#117825

Purpose

Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_YlGUT1_YlCyc1TT

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMA_Level 1Δ

Plasmid

#102704

Purpose

Mobius Assembly Level 1 Acceptor Vector Δ

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:spisPink:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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tagRFP-IMBB4-pUC

Plasmid

#170769

Purpose

test plasmid for 5RM assembly, encodes fluorescent red protein

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

tagRFP

Use

Tags

Expression

Mutation

Promoter

none

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMA_Level 2Δ

Plasmid

#102708

Purpose

Mobius Assembly Level 2 Acceptor Vector Δ

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:sfGFP:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 1

Plasmid

#102709

Purpose

Provides the Middle-to-End linker 1 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA_Auxiliary 2

Plasmid

#102710

Purpose

Provides the Middle-to-End linker 2 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA_Auxiliary 3

Plasmid

#102711

Purpose

Provides the Middle-to-End linker 3 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA_Auxiliary 4A

Plasmid

#102712

Purpose

Provides the End-to-End linker 4A which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge